conjugation of monoclonal antibodies to super paramagnetic nanoparticles is an effective method for cancer diagnosis and treatment. in this study the humanized anti her2/neu monoclonal antibody- herceptin- was conjugated to super paramagnetic iron oxide (spio) nanoparticles using edc method. the concentration of the conjugated antibodies was measured by bradford assay. the antibody-nanoparticle...

Background: Germ cell development processes are influenced by soluble factors and intercellular signaling events between them and the neighboring somatic cells. More insight into the molecular biology of the germ cell development from embryonic stem (ES) cells and investigation of appropriate factors, specifically those targeting differentiation process, is of great importance. In this study, w...

HTLV-I is the etiologic agent for adult T-cell leukemia/lymphoma (ATL) and HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Taxi, the major activator of this virus, is a 40- kDa (353 amino acid) phosphoprotein, predominantly localized in the nucleus of the host cell, which functions to trans-activate both viral and cellular promoters. Recently it has been shown that HTLV-I a...

Introduction: The tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) used extensively to measure the quantitative survival and proliferation of mammalian cells. analysis is based upon reduction MTT by metabolically active cells insoluble formazan crystals. Flavonoids are a large group natural compounds found in plants with variable phenolic structures. Flavono...

apoptosis, a physiologic mechanism to eliminate unwanted cells, is also induced by ionizing irradiation, through production of free radicals. it has been demonstrated that antioxidants such as vitamin e are able to protect cells from damage caused by free radicals. taken together we found it reasonable to make an attempt to evaluate the protective effect of vitamin e against apoptosis. the irra...

Background: Creating a new berberine liposome with high encapsulation efficiency and slow release formulation in the treatment of cancer is a new issue. Therefore, the aim of current study was to develop slow release berberine-containing nanoliposome for delivery to bone cancer cells Saos2. Materials and Methods: In this experimental study, after synthesis nanoliposomal formulation, physical p...