We developed capillary affinity electrophoresis (CAE) to analyze the molecular interaction between carbohydrate chains and proteins in solution state. A mixture of oligosaccharides derived from a glycoprotein was labeled with 8-aminopyrene-1,3,6-trisulfonate (APTS), and used as glycan library without isolation. Interaction of a carbohydrate-binding protein with each oligosaccharide in the mixtu...

Cotton grown in the Mississippi Delta often flowers in temperatures that are suboptimal for the species. High-temperature stress leads to a decrease in the photosynthetic efficiency of the plant and hinders proper growth and development. If the efficiency is hindered for a significant period of time, then the plant must repartition resources to facilitate its survival rather than for reproducti...

The glycogenic effect of propionic acid (1, 2) results from the conversion of propionate carbon into carbohydrate (3, 4) and not from an indirect stimulation of carbohydrate production from other sources. The finding (5,6) that extracts of mitochondria can convert propionate and bicarbonate to an organic acid identified as succinic acid (6) provided an explanation for this glycogenic effect. Th...

Polysaccharides are major components of macroalgal and phytoplankton biomass constitute a large fraction the organic matter produced degraded in ocean. Until recently, however, our knowledge marine polysaccharides was limited due to their great structural complexity, correspondingly complicated enzymatic machinery used by microbial communities degrade them, lack readily applied means isolate an...