A capillary isoelectric focusing (CIEF) method under denaturing condition has been developed for separation of a-keratins, highly cross-linked biomacromolecules, in horns of rhinoceros and buffalo. The a-keratins were denatured in 8 M urea and separated in the presence of ampholytes with applicable pH ranges of 3-7 or 3-10. In the preliminary results, it was demonstrated that the o~-keratins in...

N, Mangion J, Cornelis RS, Cooper CS, et al. Instability of short tandem repeats (microsateffites) in human cancer. Nature Genet 1994;6:152-6. 3. Martin F, Vairelles D, Henrion B. Automated ribosomal DNA fingerprinting by capillary electrophoresis of PCR products. Anal Biochem 1993;63:182-9. 4. Zhu M, Hansen DL, Burd S, Gannon F. Factors affecting free zone electrophoresis and isoelectric focus...

We demonstrate a two-dimensional (2D) separation microchip allowing capillary isoelectric focusing-capillary zone electrophoresis (cIEF-CZE) to be coupled to electrospray ionization mass spectrometry (ESI/MS). In the first dimension, separation is according to pI values (cIEF), while CZE occurs in the second dimension during mobilization towards the monolithically integrated ESI emitter. The se...

We employed a liquid core waveguide to image both DNA electrophoresis separations and isoelectric focusing of proteins. The utility of the system is demonstrated for DNA fragment sizing and protein separations. The system utilizes the liquid-core waveguide as an efficient window for both the excitation of separated samples and the collection of light through total internal reflectance, with an ...