Asparagus breslerianus a wild species in Iran, exhibited tolerance to salt in dry gypsum hills and dry lands. In order to check for salt tolerance thresholdvia in vitro conditions, the A. breslerianus callus was subjected to NaCl (sodium chloride) treatments. Six weeks old calli derived from male spear bud, were exposed to 0, 21.88, 43.76, 65.64, 76.58, 87.52, 109.40, 131.28, 153.16 and 175.04 ...

As a measure of testing the medicinal properties of Jatropha curcas, methanol extract obtained from both in vivo leaf and leaf derived callus were subjected to antimicrobial activity against six microorganisms, of the six different concentrations tested, the in vitro leaf callus extracts of at high concentrations (1.0 and 1.2%) inhibited the growth of Staphylococcus aureus and Pseudomonas sp. a...

The present study was carried out to evaluate the effects of light and plant growth regulators on callus induction and explant browning in date palm cultivar, Estameran in in vitro condition. The explants were pretreated by an antioxidant combination (100 mgl-1 citric acid and 150 mgl-1 ascorbic acid) for 3 days in 5 °C. Then, the  leaf explants  were cultured in Murashige and Skoog medium (MS)...

The objective of this research was in-vitro germination and callus induction of Onosma bulbotrichum (O. bulbotrichum) as a medicinal herb which belongs to Boraginaceae family. For germination, the seedswere cultured on growth regulator-free MS medium and for callus induction, seeds were sown on modified MS medium containing different concentrations of kinetin (kn)- Indole-3-acetic acid (IAA) an...

modern biotechnology and genetic engineering using techniques and special ‎laboratory tools plays an important role in the pharmaceutical industry and medicinal ‎plant propagation. among these techniques, it can be noted that a wide range of ‎elicitors are widely used for inducing metabolites at invitro culture. due to the ‎pharmaceutical properties of artichoke and its history, it has been dee...

Foliar explants of Nicotiana tabacum cv Xanthi n.c. were cultured on four different media: a basal medium, basal medium plus benzyladenine, basal medium plus 2,4-dichlorophenoxyacetic acid (2,4-D), and the basal medium containing both hormones. No differentiation or cell division occurred in leaf explants cultured on the basal medium. Addition of benzyladenine caused the formation of buds on th...

The synthetic growth regulant DPX 1840 (3,3a-dihydro-2-(p-methoxyphenyl)-8H-pyrazolo[5, 1-a]isoindol-8-one) induced callus growth and subsequent tissue differentiation on cut surfaces of decapitated Xanthi-nc tobacco plants (Nicotiana tabacum). Callus formation and organogenesis induced by DPX 1840 depended on the presence of leaves. The adventitious meristems developed into either vegetative o...