The form genus Rhizoctonia D.C. is regarded as a heterogeneous assemblage of filamentous fungal taxa that do not produce asexual spores and share a number of common features in their anamorphic states (Garcia et al. 2006). It includes several of the most devastating crop pathogens such as Rhizoctonia solani Kühn [teleomorph = Thanatephorus cucumeris (A.B. Frank) Donk]. In the absence of a perfe...

BACKGROUND The synthesis of cellulose is among the most important but poorly understood biochemical processes, especially in bacteria, due to its complexity and high degree of regulation. In this study, we analyzed both the production of cellulose by all known members of the Rhizobiaceae and the diversity of Rhizobium celABC operon predicted to be involved in cellulose biosynthesis. We also inv...

The KNR4 gene, originally isolated by complementation of a K9 killer-toxin-resistant mutant displaying reduced levels of both 1,3-beta-glucan and 1,3-beta-glucan synthase activity, was recloned from a YCp50 genomic library as a suppressor of Saccharomyces cerevisiae calcofluor-white-hypersensitive (cwh) mutants. In these mutants, which were characterized by increased chitin levels, the suppress...

Background and objective Candida albicans (C. albicans) play a significant role in oral mucosal carcinogenesis. It can be identified using various techniques in cytological smears. But, very few studies have been conducted on histopathological sections using calcofluor white M2R under fluorescent microscopy. Additionally, detection and quantification of Candida colonies and its correlation with...

Superficial mycoses caused by dermatophyte fungi are among the most common infections worldwide, yet treatment is restricted by limited effective drugs available, drug toxicity, and emergence of drug resistance. The stilbene fluorescent brightener calcofluor white (CFW) inhibits fungi by binding chitin in the cell wall, disrupting cell wall integrity, and thus entails a different mechanism of i...

Malassezia spp. are lipid-dependent yeasts that have been related to skin mycobiota and dermatological systemic diseases. Study of lipid droplets (LDs) is relevant elucidate the unknown role these organelles in gain a broader overview metabolism Malassezia. Here, we standardized two protocols for analysis LDs M. pachydermatis globosa. The first describes co-staining confocal laser-scanning fluo...

Abstract Rice false smut, caused by Ustilaginoidea virens , is one of the most destructive diseases in major world rice-producing regions. Basic leucine zipper (bZIP) proteins, which belong to an evolutionarily conserved transcription factor family and play critical roles various biological processes eukaryotes, have been previously identified U. ; however, their functions still need be further...