Sheep conceptuses from day 16 of pregnancy were cultured in the presence of [3Hlglucosamine and [14C]leucine and a high-molecular-weight glycoprotein (HMWG) secreted into the culture medium was purified by a combination of anion-exchange and gel filtration chromatography. The HMWG was found to have a molecular weight between 800 000 and 900 000 and to be highly resistant to digestion with prona...

Human hematopoietic progenitor cells express L-selectin and also express PSGL-1, a ligand for all selectins. Using a shear-based adhesion assay, a hematopoietic cell L-selectin ligand (HCLL) that is expressed on the hematopoietic cell line KG1a and on normal human hematopoietic progenitors was previously identified. To characterize the structural biology of HCLL and to define its relationship t...

Using monospecific polyclonal antisera to different parts of Chlamydia trachomatis L2 outer membrane protein 2 (Omp2), we show that the protein is localized at the inner surface of the outer membrane. Omp2 becomes immunoaccessible when Chlamydia elementary bodies are treated with dithiothreitol, and protease digestions indicate that Omp2 has a possible two-domain structure.

the effect of ca, zn and b foliar application (five g l-1) and their mixed applications as well as two harvest dates (1st and 15st of august) on ppo activity of two commercial asian pears (‘ks’9 and ‘ks’13) fruits at the time of harvest and during storage were investigated. results showed that the ppo activity increased from harvest time to three months after storage and then decreased. during ...

One of the most intriguing questions in modern enzymology is whether enzyme dynamics evolved to enhance the catalyzed chemical transformation. In this study, dihydrofolate reductase, a small monomeric protein that catalyzes a single C-H-C transfer, is used as a model system to address this question. Experimental and computational studies have proposed a dynamic network that includes two residue...

INTRODUCTION Chondrocytes maintain a balance between catabolic and anabolic activities in order to provide cartilage tissue with its characteristic concentrations of extracellular matrix molecules such as glycosaminoglycans (GAGs) and collagen. Disruption of this balance may have implications for disease progression and loss of tissue properties. As demonstrated in a recent study [1], when the ...

organophosphorus compounds are highly toxic chemicals that have application in production of warfare agents and agricultural pesticides. detoxification of areas that have been exposed to these types of materials is of vital importance and several approaches have been used for this purpose. biodegradation of organophosphates is one of these approaches that is in the center of attention due to it...

Aim: The study compares and evaluates enzymatic gel Brix 3000â Polymer bur (SS White) for caries excavation in primary molars terms of two parameters such as pain reaction the time taken excavation. Method: 60 children aged 7-9 years who require removal were recruited randomly allocated into groups: Group-A: Enzymatic (Brix 3000)â, Group-B: Smart burs White polymer burs). objectives evaluated w...

Partial proteolytic fragmentation of the two chick oviduct progesterone receptor subunits was used to identify structural features shared by the two proteins. Both subunits can be photoaffinity labeled at their hormone-binding sites (Birnbaumer, M., Schrader, W. T., and O'Malley, B. W. (1983) J. Biol. Chem. 258, 1637-1644) using the radioactive steroid [methyl-3H] 17 alpha, 21-dimethyl-19-nor-p...