The effect of the digestive microflora on oral tolerance to ovalbumin was studied by using axenic (germfree) and conventional C3H/HeJ mice. In contrast to reported results of studies with sheep erythrocytes, oral administration of ovalbumin induced tolerance in axenic mice, but the maintenance of tolerance was found to be of shorter duration than was with conventional mice. These data indicate ...

The length and diameter of enterocyte microvilli at mid-villus position were measured on electron-micrographs. The duodenum, jejunum and ileum of axenic (germfree) and holoxenic (conventional) inbred rats fed the same diet have been studied. The microvilli were significantly shorter in all these intestinal regions when the microflora was present. The decrease in microvillus length (due to the p...

In vitro morphogenic response of mature seed embryo-derived callus cultures Chonemorpha fragrans was studied using solid and liquid Murashige Skoog medium amended with cytokinins or their combinations naphthalene acetic acid at 0.5 mg L-1. The plant growth regulators (PGRs) combination concentrations tested could not stimulate organogenesis after three subcultivations the on same PGRs-amended w...

Metagrowth is a new type of knowledge base developed to guide the experimental studies of culture conditions of obligate parasitic bacteria. We have gathered biological evidences giving possible clues to the development of the axenic (i.e. 'cell-free') growth of obligate parasites from various sources including published literature, genomic sequence information, metabolic databases and transpor...

Algal growth is often inhibited in full-strength anaerobic digestate. The objective of this study was to investigate conditions under which digestate pretreatment using bacteria effective promoting algal growth, nutrient removal, and favorable changes biomass composition. Batch culture experiments were carried out low- high-strength municipal sludge digestate, two algae strains varying sensitiv...

Amastigogenesis occurs first when metacyclic trypomastigotes from triatomine urine differentiate into amastigotes inside mammalian host cells and a secondary process when tissue-derived trypomastigotes invade new cells and differentiate newly to amastigotes. Using scanning electron microscopy, we compared the morphological patterns manifested by trypomastigotes and metacyclic forms of Trypanoso...

Galdieria sulphuraria is considered one of the most promising microalgae for food applications. In this study, we compared two strains G. cultivated autotrophically and mixotrophically over 35 days in pilot-scale photobioreactors under nonsterile conditions. The low pH (<1.9) used cultivation successfully prevented microbial contamination. had similar autotrophic mixotrophic biomass productivit...