We conducted a comprehensive genomic analysis of the temporal response of yeast to anaerobiosis (six generations) and subsequent aerobic recovery ( approximately 2 generations) to reveal metabolic-state (galactose versus glucose)-dependent differences in gene network activity and function. Analysis of variance showed that far fewer genes responded (raw P value of <or=10(-8)) to the O(2) shifts ...

Wild riverine fishes are known to rely on burst swimming to traverse hydraulically challenging reaches, and yet there has been little investigation as to whether swimming anaerobically in areas of high flow can lead to delayed mortality. Using acoustic accelerometer transmitters, we estimated the anaerobic activity of anadromous adult sockeye salmon (Oncorhynchus nerka) in the tailrace of a div...

In contrast to previous steady-state analyses of the O(2)-responsive transcriptome, here we examined the dynamics of the response to short-term anaerobiosis (2 generations) in both catabolite-repressed (glucose) and derepressed (galactose) cells, assessed the specific role that Msn2 and Msn4 play in mediating the response, and identified gene networks using a novel clustering approach. Upon shi...

Previous investigations of the effect of anaerobiosis on the metabolism of glucose have been carried out with mammalian tissues. Walaas and Walaas (1) found no difference in the rate of glucose uptake by rat hemidiaphragms incubated in Ringerphosphate solution with either oxygen or nitrogen as the gas phase. The addition of insulin exerted an effect on glucose uptake only during the first few m...

In the presence of nitrate, the major anaerobic respiratory pathway includes formate dehydrogenase (FDH-N) and nitrate reductase (NAR-A), which catalyze formate oxidation coupled to nitrate reduction. Two aerobically expressed isoenzymes, FDH-Z and NAR-Z, have been recently characterized. Enzymatic analysis of plasmid subclones carrying min 88 of the Escherichia coli chromosome was consistent w...