anopheles fluviatilis complex is known to be a vector of malaria in iran. since mosquitoes of this species cover a wide geographical range in iran, they might have evolved into different separated populations. random amplified polymorphic dna polymerase chain reaction (rapd-pcr) assay was used to differentiate geographic populations of this species. dna was extracted from individual mosquitoes ...

Research to determine patterns of genetic differentiation among and within field populations of Crom.um quercuum f. sp.firsifome using RAPD markers is currently underway in the molecular genetics laboratory at the Southern Institute of Forest Genetics. Fungal tissue was collected as a drop of spermatia or scrapings of a discrete hymenium fiom a single gall on each tree sampled at a location. Co...

Methods for identifying isolates of various pathogenic bacteria by DNA fingerprinting with random primers (RAPD) have been described recently. In these methods many primers are screened and the primers that generate the most informative DNA pattern are selected. A new strategy that simplifies the primer selection process for RAPD fingerprinting has been developed in our laboratory. In this appr...

Three controlled crosses were carried out involving Mentha arvensis and Mentha spicata [M. spicata CIMAP/C30 x M. spicata CIMAP/C33 (cv. Neera); M. arvensis CIMAP/C18 x CIMAP/C17 (cv. Kalka); and M. arvensis CIMAP/C17 x M. spicata CIMAP/C33]. The parents were subjected to random amplified polymorphic DNA (RAPD) analysis with 80 primers, and polymorphic primers were tested for detecting coinheri...

genetic diversity of 36 genotypes of dwarf and semi-dwarf apple rootstocks along with seven iranian dwarf rootstocks were investigated by use of rapd dna markers. a set of 100 random primers was utilized in pcr reaction from which 10 primers resulted in good amplification with high polymorphism. among primers, 10 selected rapd primers produced 160 bands, in which, 136 were polymorphic. cluster ...

In this work, we described a new approach for the isolation of a species-specific probe for the Eimeria media parasite of the rabbit based on the use of the random amplified polymorphic DNA (RAPD) technique. A specific fragment of 800 bp of the studied species was isolated after RAPD and then cloned and DIG-radiolabeled. After dot-blotting, we observed that this probe was specific for E. media....

Drug susceptibility profile of 53 Mycobacterium tuberculosis clinical isolates was studied by conventional solid and liquid culture methods. Forty three isolates were drug resistant to at least one drug and 10 were susceptible. The strain identification was performed by PCR amplification of mtp40 gene and strain subtyping was performed by Double Repetitive Element-PCR (DRE-PCR), IS6110 outward ...

Staphylococcus aureus is a known major cause of foodborne illnesses, and milk and dairy products are often contaminated by enterotoxigenic strains of this bacterium. In the present study, 122 S. aureus isolates collected from different dairy products were characterised by phenotypic properties, by the distribution of genes encoding staphylococcal enterotoxins (sea, sec, sed, seg, seh, sei, sej,...