Current hypotheses suggest that both the plasmin system and metalloproteinases are involved in tumor invasion of basement membrane. In this study, we demonstrate that plasmin can directly degrade native and denatured type IV collagen in solution as well as in tissue sections. Tumor cell lines secreted plasminogen activators into culture supernatants that activated exogenous plasminogen to degra...

Department of Microbiology, University of Leeds, Leeds LS2 9JT, UK Incubation of Aspergillus fumigatus NCPF 2140 in growth medium containing 1 O/ O chitin as sole carbon source led to induction of specific extracellular chitinolytic activity of 1-5 pmol GlcNAc released min'l (mg protein)'l. The effect was repressed by the inclusion of GlcNAc in the medium, indicating regulation by a negative fe...

PG1, the major endopolygalacturonase of the vascular wilt pathogen Fusarium oxysporum, was secreted during growth on pectin by 10 of 12 isolates belonging to seven formae speciales, as determined with isoelectric focusing zymograms and sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels. A Southern analysis of genomic DNA and PCR performed with gene-specific primers revealed that the...

1. The frequency of occurrence, under defined conditions, of the different human pepsins in the gastric juices of 50 normal subjects was investigated by agar-gel electrophoresis. 2. From a total of eight proteolytic zones located in the zymograms, no significant differences of occurrence existed between the sexes, or between subjects with or without gastric symptoms. 3. Two zones, numbered 3 an...

C4 grasses are common species in rangelands around the world and represent an attractive option for second-generation biofuel production. Although they display high polysaccharide content reach great levels of biomass accumulation, there is a major technical issue to be addressed before can used bioethanol industrial production: lignin removal. Concerning this, Pycnoporus Ganoderma fungal gener...

The study of fish digestive biochemistry is essential to understand factors that affect the net efficiency food transformation and growth, therefore aquaculture profitability. aim present was assess activity functional characteristics key enzymes in juveniles greater amberjack ( Seriola dumerili ), as well possible modulation their relative importance by water temperature. For that, a combinati...