glycosyltransferase. the enzyme as a cyclodextrin thermosulfurigenes EM1 and reclassification of alpha-amylase of Thermoanaerobacterium Cyclodextrin formation by the thermostable

A protease producing microorganism was isolated from soil collected from a detergent industry and identified as Bacillus species. Isolate K-30 produced thermostable alkaline protease utilizing rice bran. The optimum conditions for protease activity was 55°C at pH 9 with 4% inoculum in the medium containing 1% rice bran after 96 h of incubation. Beef extract, tryptone and yeast extract were good...

Lignocellulose is the most abundant carbohydrate source in nature and represents an ideal renewable energy source. Thermostable enzymes that hydrolyze lignocellulose to its component sugars have significant advantages for improving the conversion rate of biomass over their mesophilic counterparts. We review here the recent literature on the development and use of thermostable enzymes for the de...

gene (tdh) and the thermostable direct hemolysin-related hemolysingene (blh) of Vibrio parahaemolyticus by polymerase chain reaction. Mol. Cell. Probes, 6, 477-487. 3. Sugiyama, A., Nakano, Y., Iwade, Y., Yamauchi, A., Nakayama, 0., Matsumoto, T., Kou, Y., Ito, T., Nishinaka, T. and Shoji, T. (1 999): Isolation of thermostable direct hemolysin-producing Vibrio parahaemolyticusfrom food implicat...

Selective media for Vibrio parahaemolyticus, BTB-Teepol agar and modified arabinose-ammonium sulfate-cholate agar, were modified for use in immunological detection of the thermostable direct hemolysin produced by this organism. The modified BTB-Teepol agar and modified arabinose-ammonium sulfate-cholate agar were both found to be useful for the modified Elek test and immunohalo test with antise...

In a recent edition of the Journal of Clinical Microbiology, Qian et al. described the sensitivity and specificity of the direct tube coagulase (DTC) test for the presumptive identification of Staphylococcus aureus directly from positive blood culture bottles from which Gram stains were consistent with staphylococci (7). The reported sensitivities of 34% at 2 h of incubation and 65% at 4 h of i...

Here we describe a half-semester biochemistry laboratory course based on the expression, isolation, purification, and biochemical characterization of RecJ nuclease. The students transformed expression plasmid thermostable nuclease into host bacteria Escherichia coli BL21(DE3), to induce recombinant by IPTG. expressed was purified from cell lysates broken with sonication Ni-affinity chromatograp...

Robust vaccines are required to protect village chickens against Newcastle disease. Thermostable vaccines derived from avirulent Australian strains of Newcastle disease virus (strains V4 and I-2) have proved successful for this purpose. These vaccines have been developed through ACIAR projects and have been adopted by other international aid agencies. It is now possible to offer integrated work...

We report the isolation, cloning and expression, in Bacillus subtilis, of the gene coding for thermolysin, a thermostable metalloprotease which is produced by Bacillus thermoproteolyticus Rokko. The nucleotide sequence has revealed that, like neutral proteases produced by other members of the Bacillus species, thermolysin is probably produced as a preproenzyme carrying a typical N-terminal memb...