Red blood cells can be preserved by freezing using either intraor extracellular agents. The most common intracellular agent, glycerol, is currently employed in general use and provides clinically acceptable red cells following post-thaw washing (13). Extracellular agents do not enter the cell during freezing. Therefore, red cells preserved with these agents may be administered without post-thaw...

An endoglycosidase which cleaves heparin and heparan sulfate was isolated from outdated human platelets by freeze-thaw solubilization, heparin-Sepharose chromatography, DEAE-cellulose chromatography, hydroxylapatite chromatography, octyl-agarose chromatography, concanavalin A-Sepharose chromatography, and Sephacryl S-200 gel filtration. The overall extent of purification of the platelet heparit...

BACKGROUND Best practice when performing culture-independent microbiological analysis of sputum samples involves their rapid freezing and storage at -80°C. However, accessing biobanked collections can mean that material has been passed through repeated freeze-thaw cycles. The aim of this study was to determine the impact of these cycles on microbial community profiles. METHODS Sputum was coll...

Assisted reproductive techniques might prove themselves useful tools in producing buffaloes free of specific diseases (BFSD), which are in demand in South Africa. Freezing protocols for African buffalo semen must not only result in good post-thaw qualities, but must also be practical. Epididymal sperm from six mature African buffalo bulls was collected, diluted with three different semen extend...

The cold climate is a favorable parameter for the development of tension cracks and decrease of rock brittleness. Therefore, this paper attempts to investigate the Hamekasi porous limestone in order to predict the brittleness indices during freeze-thaw cycles. The freeze–thaw test was executed for one cycle including 16 h of freezing, and 8 h of thawing. The geo mechanical properties and brittl...

The NASA Soil Moisture Active and Passive (SMAP) mission is dedicated to measurement of global soil moisture and boreal land surface freeze/thaw state [1]. The satellite will carry radar (active) and radiometer (passive) L-band instruments that will perform simultaneous and coincident measurements of the Earth’s surface. The combination of data from the two instruments will allow unprecedented ...

Remotely sensed estimates of snow thaw offer the potential of more complete spatial coverage across remote, undersampled areas such as the terrestrial Arctic drainage basin. We compared the timing of spring thaw determined from approximately 25 km resolution daily radar backscatter data with observed daily river discharge time series and model simulated snow water content data for 52 basins (50...

Ferrous and ferric sulphates and (oxy)hydroxides can grow on pyrite surfaces and slow oxidation rates. However, in northern climates, repeated freeze/thaw cycles can cause cracks, channeling and exposure of new surfaces. In the present study, weathering in a repeated freeze/thaw experiment was compared to a regular humidity cell experiment. Introductory results (after 17 weeks) show very small ...

background: an effect of cryopreservation on human sperm is sublethal cryodamage, in which cell viability post-thaw is lost more rapidly at later times than in fresh cells. objective:  this study examined whether the addition of an antioxidant to cryopreservation medium could improve the post-thaw parameters and evaluation of sperm chromatin quality of cryopreserved human spermatozoa from men w...