BACKGROUND Sodium arsenite can impair male reproductive function by inducing oxidative stress. Silymarin is known as a potent antioxidant. OBJECTIVE This study was performed to investigate if silymarin can prevent the adverse effect of sodium arsenite on ram sperm viability, motility and mitochondrial membrane potential. MATERIALS AND METHODS Epidydimal spermatozoa obtained from ram were di...

BACKGROUND Globozoospermia is a severe form of teratozoospermia (incidence < 0.1%) in infertile men that is characterized by round headed sperm and acrosomeless in semen. OBJECTIVE To compare the semen parameters, protamine deficiency, and apoptosis in ejaculated spermatozoa between globozoospermic and normozoospermic men. MATERIALS AND METHODS Thirty six semen samples were divided into two...

AIM To evaluate the fertilization competence of spermatozoa from ejaculates and testicle when the oocytes were matured in vitro following intracytoplasmic sperm injection (ICSI). METHODS Fifty-six completed cycles in 46 women with polycystic ovarian syndrome were grouped according to the semen parameters of their male partners. Group 1 was 47 cycles that presented motile and normal morphology...

background: exposure to arsenic is associated with impairment of male reproductive function by inducing oxidative stress. silymarin with an antioxidant property scavenges free radicals. objective: the aim of this study was to investigate if silymarin can prevent the adverse effects of sodium arsenite on ram sperm plasma membrane and acrosome integrity. materials and methods: ram epidydimal sper...

In this study, the formation of hydrogen peroxide (H2O2) by chilled goat spermatozoa was measured. Furthermore, the effects of dead spermatozoa on motility characteristics were studied. Fresh collected ejaculates from five Shami bucks were centrifuged and virtually all seminal plasma was removed. A part of the collected spermatozoa was killed by two ways: the first by repe...

Background: Epididymal spermatozoa have been used for production of offspring from genetically important animals after they are dead. To attain optimal fertility with epididymal spermatozoa, they should be preserved in a suitable medium in 37°C. Preservation media provides requirement for spermatozoa and affects its motility, viability, and fertility. Sucrose is one of the important components ...

The assessment of spermatozoa motility is one of the most often used parameters for semen evaluation. The study was conducted at Poultry Research Station, Madhavaram Milk Colony, Chennai600 051, to  nd the effect of different semen diluents and dilution ratio on per cent spermatozoa motility of pooled guinea fowl semen at 5o C under short term storage(0, 3 and 6 Hours). Irrespective of the sto...

Sperm DNA integrity is essential for accurate transmission of genetic material to offspring. Fragmentation of genomic DNA is an initial hallmark of apoptosis (programmed cell death). The aim of this study was to determine sperm nuclear DNA integrity and mitochondrial function, to quantify possible apoptosis and to investigate any relationship between these parameters. Semen samples (n = 25) wer...

In this study, we evaluated the potential effect of the method of recovery (artificial vagina or electroejaculation) on the production and quality of Guirra ram spermatozoa cryopreserved for the possible constitution of a sperm bank. In order to address this question, we evaluated the effect of semen collection method on fresh semen quality parameters, including: volume, concentration, producti...

Abstract Recent reports suggest that mobile phone radiation may diminish male fertility. However, the effects of this radiation on human spermatozoa are largely unknown. The present study examined effects of the radiation on induction of apoptosis-related properties in human spermatozoa. Ejaculated, density-purified, highly motile human spermatozoa were exposed to mobile phone radiation at spec...