background: today a large number of bacterial amylases are available commercially in industry. the goal of the present study was purification and biochemical characterization of an extracellular thermostable alkaline α-amylase from the novel moderately halophilic, bacillus persicus from the aran-bidgol, iran.   methods : purification of enzyme, was carried out by acetone precipitation, ultrafil...

BACKGROUND: Clostridium septicum has played a significant role as a causative agent of many acute fetal diseases in man and animals. Alpha- toxin is the main factor in the pathogenesis of C. septicum with hemolytic, necrotic and lethal activities. OBJECTIVES: The study was designed to evaluate alpha-toxin purification and antibody production rate against a local strain of C. septicum NH2 which ...

background and objectives : streptokinase is used clinically as an intravenous thrombolytic agent for the treatment of acute myocardial infarction and is commonly prepared from cultures of streptococcus equisimilis strain h46a. the objective of the present study was the production of streptokinase from strain h46a and purification by chemical reduction method. materials and methods: the rate of...

In this study, multi wall carbon nanotubes (MWCNTs) were synthesized by Chemical vapor deposition (CVD) method using two different catalysts (Fe nanoparticles) and (Ni nanoparticles) with  two different substrates: Quartz and Alumina. Acetylene gas was used as a carbon source and Argon as a carrier gas at different temperatures and different times to study the effects of these parameters on CNT...

In this study, multi wall carbon nanotubes (MWCNTs) were synthesized by Chemical vapor deposition (CVD) method using two different catalysts (Fe nanoparticles) and (Ni nanoparticles) with  two different substrates: Quartz and Alumina. Acetylene gas was used as a carbon source and Argon as a carrier gas at different temperatures and different times to study the effects of these parameters on CNT...

bacillus thuringiensis (bt) has been extensively used in biopesticidal formulations due to its safe environmental and human health records. the widespread use of bt is often challenged by production as well as formulation costs which are in direct contact with downstream processing, i.e. the separation/purification step. downstream separation/purification efficacy governs the marketability of a...

egg-laying hens were immunized with the echis carinatus venom and the resulting antibodies were extracted from egg yolk by four different purification methods. the chicken egg yolk antibodies were purified by the water dilution method, polyethylene glycol (peg) and ammonium sulphate precipitation method, chloroform extraction and the lithium sulphate precipitation method. these methods were com...

background: production of tissue plasminogen activator protein (t-pa) in prokaryotes systems has many problems such as the lack of active protein production, multiple purification steps, and renaturation process which has been shown to be costly and time-consuming. methods: in this study, reteplase which is the nonglycosylated active domain of t-pa was used to transform top10 escherichia coli (...