BACKGROUND Various approaches have been utilized in attempting to cryopreserve oocytes, beginning with slow cooling and more recently the advent of technique of vitrification. Now it seems that oocyte cryopreservation is no longer an experimental technique and it is being increasingly utilized in clinics around the world. As successful outcome in oocyte cryopreservation can be assessed by survi...

Abstract BACKGROUND The health benefits provided by fruit mean that there is continuous interest in offering consumers new products to stimulate its consumption. To this end, dehydrated snacks may be an interesting option. In study, we evaluated the impact of freezing rate (slow and high), shelf temperature (40 50 °C), working pressure (5 100 Pa) on perception acceptability a freeze‐dried orang...

Embryos and oocytes were first successfully cryopreserved more than 30 years ago, when Whittingham et al. and Wilmut separately described that mouse embryos could be frozen and stored at -196 degrees C and, a few years later, Parkening et al. reported the birth of live offspring resulting from in vitro fertilization (IVF) of cryopreserved oocytes. Since then, the use of cryopreservation techniq...

freeze-thaw is one of the most powerful weathering agents that may cause a rapid change in the initial engineering properties of stones, and limit their durability. freeze-thaw induces stress over the pores’ surface of stones. consequently, stone durability is closely related to its engineering and physical properties. the purpose of this study is estimating the engineering properties of deteri...

BACKGROUND The aim of this study was to determine whether the use of visual freeze indicators on vaccines would assist health care providers in identifying vaccines that may have been exposed to potentially damaging temperatures. METHODS Twenty-seven sites in Connecticut involved in the Vaccine for Children Program participated. In addition to standard procedures, visual freeze indicators (FR...

Pulsed dipolar ESR spectroscopy, DEER and DQC, require frozen samples. An important issue in the biological application of this technique is how the freezing rate and concentration of cryoprotectant could possibly affect the conformation of biomacromolecule and/or spin-label. We studied in detail the effect of these experimental variables on the distance distributions obtained by DEER from a se...

Many limno-terrestrial tardigrades live in unstable habitats where they experience extreme environmental conditions such as drought, heat and subzero temperatures. Although their stress tolerance is often related only to the anhydrobiotic state, tardigrades can also be exposed to great daily temperature fluctuations without dehydration. Survival of subzero temperatures in an active state requir...

In the slow-wave (SW) state, the vast majority of cortical neurons exhibit mostly synchronized oscillatory activity. In this study, we examined the right-left hemispheric difference in slow-wave timings in urethane-anesthetized mice. We found that interhemispheric cross-correlograms of local field potentials (LFPs) peaked asymmetrically. Double in vivo whole-cell patch-clamp recordings also rev...

Mammalian fast and slow twitch skeletal muscles are compared by freeze-fracture, thick and thin sectioning, and histochemical techniques using conventional and high voltage electron microscopy. Despite gross morphological differences in endplate structure visualized at relatively low magnifications in this sections, rat extensor digitorum longus (EDL) (fast twitch) and soleus (slow twitch) fibe...

The growth curves of strains belonging to genera Candida and Saccharomyces grown through surfaced and submerged cultivation and their survival after lyophilization have been studied. Conclusions of practical importance have been drawn. It was found out that three-day-old cultures cultivated in solid medium possessed maximal resistance to freeze-drying. Nevertheless the cultures obtained by subm...