We have developed a method for labeling retinal ganglion cells in neonatal rats by retrograde transport of the fluorescent dye, True Blue (TB), injected into the optic chiasm. Following proteolytic dissociation of labeled retinas into single cells, the labeled cells could be enriched 50- to 100-fold by centrifugation in a 5%/10% metrizamide gradient. When plated in Ham's F-10 medium in the pres...

b a ckground: the role of lectins is important in interaction between pathogens and mosquito vectors. this study was performed to identify agglutinin activities of protein molecules on the midgut of culex pipiens . m e t hods: c u lex pipiens was reared in insectray condition and the midguts of males and females (blood fed and un- fed) were dissected separately in tris-hcl buffer. the extracts ...

A new serological technique was introduced into blood grouping of blood stains and other test specimens. In this method, latex-adsorbed agglutinin was prepared by mixing polyvinyl-toluene latex particles of 2.015ƒÊ in diameter with antiserum. Accurate blood grouping was possible with the agglutinin-adsorbing latex particles directly even on a small piece of blood or saliva-stained fibrils in on...

Antipneumococcus serum under certain conditions apparently inhibits or retards the metabolic activities of the homologous pneumococcus. Antipneumococcus serum exhausted of its agglutinin content possesses no inhibitory properties. The degree of inhibitory action of antipneumococcus serum parallels its agglutinating power. No evidence has been found to indicate that the inhibition of the metabol...

The microagglutination technique without centrifugation was more effective than centrifugation of the standard tube test for increasing Brucella agglutinin titers of specimens with a titer greater than or equal to 160 but was less effective than centrifugation of the standard tube test for specimens with a titer less than 160.

Recombinant equine luteinizing hormone/chorionic gonadotropin (eLH/CG) was expressed in Mimic insect cells, that are commercial stably transformed Spodoptera frugiperda (Sf9) cells expressing five mammalian genes encoding glycosyltransferases involved in the synthesis of complex-type monosialylated N-glycans. We previously showed that it exhibited no in vivo bioactivity although expressing full...

Recombinant equine luteinizing hormone/chorionic gonadotropin (eLH/CG) was expressed in MimicTM insect cells, that are commercial stably transformed Spodoptera frugiperda (Sf9) cells expressing five mammalian genes encoding glycosyltransferases involved in the synthesis of complex-type monosialylated N-glycans. We previously showed that it exhibited no in vivo bioactivity although expressing fu...

intradermal injection of autologous serum and plasma elicit a cutaneous reactivity in almost 45-60% of patients with chronic idiopathic urticaria (ciu).   this reactivity is associated with the presence of auto antibodies against ige or ige receptors. this study was carried out to compare the cutaneous reactivity of autologous serum and plasma skin tests in a series of patients with ciu for dia...

Agglutination by monovalent serum is the method customarily employed to establish the type diagnosis of meningococcus. The test is simple and, in certain of its modifications, quite rapid. It is even applied by some bacteriologists directly to any gram-negative diplococci encountered in primary culture of the upper respiratory tract before their species has been determined by sugar fermentation...

objective: to evaluate the capability of ascitic fluid dipstick results for ph, glucose, and protein in order to predict a low serum-ascites albumin gradient (saag) at the bedside of the patient in the emergency department (ed). methods: this prospective cross-sectional study was conducted during one year in the ed of afzalipour hospital in kerman, iran. all patients with diagnostic or therapeu...