Partially degenerate oligonucleotides based on peptide sequence were used to isolate cDNA to a 63-kDa bovine brain calmodulin-stimulated phosphodiesterase (CaM-PDE) isozyme. A 412-base pair polymerase chain reaction fragment was obtained and used along with the oligonucleotides to isolate several cDNAs each encoding sequence identical to known peptide sequences from the 63-kDa CaM-PDE. The larg...

Nowadays, smartphones have started being used as a tool to collect and spread politically sensitive or activism information. The exposure of the possession of such sensitive data shall pose a risk in severely threatening the life safety of the device owner. For instance, the data owner may be caught and coerced to give away the encryption keys so that the encryption alone is inadequate to mitig...

New methods for solving certain types of PDE-constrained optimization problems are presented in this thesis. The approach taken is to augment state-of-the-art PDE methods. The PDE variables and the optimization variables are solved for simultaneously. This impacts the PDE method by changing the core from solving a system of nonlinear equations to that of finding a nonlinear saddle point. This i...

Introduction: Phosphocholine (PC) and total choline-containing compounds (tCho = glycerophosphocholine (GPC) + PC + free choline (Cho)) are elevated in human breast cancers, as demonstrated by numerous H magnetic resonance (MR) spectroscopy (MRS) studies [1, 2]. A switch from high GPC and low PC to low GPC and high PC characterizes the choline metabolite profile of breast [3] and ovarian [4] ca...

Estimation of L norms of Fourier multipliers is known to be hard. It is usually connected to some interesting types of PDE, see several such PDE for several Fourier multipliers on the line in a recent paper of Kalton and Verbitsky [13]. Sometimes, but much more rarely, one can establish sharp L estimates for Fourier multipliers in several variables. Riesz transforms are examples of success. The...

Many of the central equations of mathematical physics, the nonlinear wave equation, the nonlinear Schrödinger equation, the Euler equations for free surfaces, can be posed as Hamiltonian systems with infinitely many degrees of freedom. In a neighborhood of an equilibrium, the linearized equations are those of a harmonic oscillator and thus solutions exhibit periodic and quasi-periodic motion. T...

Phophodiesterase (PDE) isoenzyme profiles of human cell preparations and tissues have been analysed by a semiquantitative method using selective PDE inhibitors and activators. Neutrophils, eosinophils and monocytes contain PDE IV exclusively. Lymphocytes, alveolar macrophages and endothelial cells contain PDE IV and PDE III, and in addition, PDE I is measured in macrophages and PDE II in endoth...

In this article, we are interested in the Gevrey properties of formal power series solution time partial differential equations with a polynomial semilinearity and analytic coefficients at origin $${\mathbb {C}}^{n+1}$$ . We prove particular that inhomogeneity equation together s-Gevrey for any $$s\ge s_c$$ , where $$s_c$$ is nonnegative rational number fully determined by Newton polygon associ...

In rod outer segments the light activation of cGMP phosphodiesterase (PDE alpha beta gamma 2) is accomplished by removal of the gamma inhibitory subunit (PDE gamma) from the PDE alpha beta catalytic subunits. A light activation of the inositol signaling pathway also occurs, but there is little information linking these two signal transduction pathways. Here we report that protein kinase C (PKC)...

Four cyclic nucleotide phosphodiesterase (PDE) activities were separated from low-speed supernatants of homogenates of human cardiac ventricle by DEAE-Sepharose chromatography, and designated PDE I-PDE IV in order of elution with an increasing salt gradient. PDE I was a Ca2+/calmodulin-stimulated activity, and PDE II was an activity with a high Km for cyclic AMP which was stimulated by low conc...