نتایج جستجو برای: reverse transcription

تعداد نتایج: 334537  

Journal: :Nucleic acids research 1997
U Koppelhus V Zachar P E Nielsen X Liu J Eugen-Olsen P Ebbesen

We have tested the inhibitory potential of peptide nucleic acid (PNA) on in vitro reverse transcription of the HIV-1 gag gene. PNA was designed to target different regions of the HIV-1 gag gene and the effect on reverse transcription by HIV-1, MMLV and AMV reverse transcriptases (RTs) was investigated. We found that a bis-PNA (parallel antisense 10mer linked to antiparallel antisense 10mer) was...

Journal: :Journal of virology 2000
N A Kootstra B M Zwart H Schuitemaker

Previously, we and others have demonstrated that the process of reverse transcription of human immunodeficiency virus type 1 (HIV-1) is disturbed in nondividing macrophages and quiescent T lymphocytes. Here we analyzed which phase of the cell cycle in macrophages is crucial for early steps in the HIV-1 replication cycle. HIV-1 Ba-L-inoculated macrophages arrested early in the G(1) phase by n-bu...

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