نتایج جستجو برای: reprogramming

تعداد نتایج: 12767  

2010
Jinnuo Han Perminder S. Sachdev Kuldip S. Sidhu

Reprogramming of somatic cells to different extents has been reported using different methods. However, this is normally accompanied by the use of exogenous materials, and the overall reprogramming efficiency has been low. Chemicals and small molecules have been used to improve the reprogramming process during somatic cell nuclear transfer (SCNT) and induced pluripotent stem (iPS) cell generati...

2016
Sheng Zhang Xin Chen Fang Wang Xinglan An Bo Tang Xueming Zhang Liguang Sun Ziyi Li

DNA methylation reprogramming plays important roles in mammalian embryogenesis. Mammalian somatic cell nuclear transfer (SCNT) embryos with reprogramming defects fail to develop. Thus, we compared DNA methylation reprogramming in preimplantation embryos from bovine SCNT and in vitro fertilization (IVF) and analyzed the influence of vitamin C (VC) on the reprogramming of DNA methylation. The res...

Journal: :Stem cells 2013
Jiao Jiao Yujiao Dang Yuanyuan Yang Rui Gao Yu Zhang Zhaohui Kou Xiao-Fang Sun Shaorong Gao

Leukemia inhibitory factor and bone morphogenetic protein signaling pathways play important roles in maintaining the self-renewal of mouse embryonic stem cells (ESCs). In contrast, the supplementation of fibroblast growth factor 2 (FGF2) in culture promotes mouse ESC differentiation. It has been proposed that factors that are adverse for maintaining the self-renewal of ESCs might play detriment...

2017
Marta Martin-Lopez Laura Maeso-Alonso Sandra Fuertes-Alvarez Diego Balboa Virginia Rodríguez-Cortez Jere Weltner Inmaculada Diez-Prieto Andrew Davis Yaning Wu Timo Otonkoski Elsa R Flores Pablo Menéndez Margarita M Marques Maria C Marin

The generation of induced pluripotent stem cells (iPSCs) by somatic cell reprogramming holds great potential for modeling human diseases. However, the reprogramming process remains very inefficient and a better understanding of its basic biology is required. The mesenchymal-to-epithelial transition (MET) has been recognized as a crucial step for the successful reprogramming of fibroblasts into ...

2016
Sandy S.C. Hung Nicole J. Van Bergen Stacey Jackson Helena Liang David A. Mackey Damián Hernández Shiang Y. Lim Alex W. Hewitt Ian Trounce Alice Pébay Raymond C.B. Wong

Reprogramming of somatic cells into a pluripotent state is known to be accompanied by extensive restructuring of mitochondria and switch in metabolic requirements. Here we utilized Leber's hereditary optic neuropathy (LHON) as a mitochondrial disease model to study the effects of homoplasmic mtDNA mutations and subsequent oxidative phosphorylation (OXPHOS) defects in reprogramming. We obtained ...

2016
Shaohua Zhu Jiani Cao Hongyan Sun Kun Liu Yaqiong Li Tongbiao Zhao

Pluripotent stem cells (PSCs), including embryonic and induced pluripotent stem cells (iPSCs), show atypical cell cycle regulation characterized by a high proliferation rate and a shorter G1 phase compared with somatic cells. The mechanisms by which somatic cells remodel their cell cycle to achieve the high proliferation rate of PSCs during reprogramming are unclear. Here we identify that the I...

Journal: :JDIM 2011
Mande Xie Guoping Zhang

At present, the online network reprogramming faces two challenges: 1) How to save the energy consumed during the code dissemination; and 2) How to enhance the security of online network reprogramming. In this paper, we modify the work flow of online reprogramming system and separates an alone reprogramming component from the whole program image which need not be transmitted in order to reduce t...

2013
Dong Wook Han Natalia Tapia Marcos J. Araúzo-Bravo Kyung Tae Lim Kee Pyo Kim Kinarm Ko Hoon Taek Lee Hans R. Schöler

Epiblast stem cells (EpiSCs) and embryonic stem cells (ESCs) differ in their in vivo differentiation potential. While ESCs form teratomas and efficiently contribute to the development of chimeras, EpiSCs form teratomas but very rarely chimeras. In contrast to their differentiation potential, the reprogramming potential of EpiSCs has not yet been investigated. Here we demonstrate that the epibla...

2014
Rodrigo L. dos Santos Luca Tosti Aliaksandra Radzisheuskaya Isabel M. Caballero Keisuke Kaji Brian Hendrich José C.R. Silva

The Nucleosome Remodeling and Deacetylase (NuRD) complex is essential for embryonic development and pluripotent stem cell differentiation. In this study, we investigated whether NuRD is also involved in the reverse biological process of induction of pluripotency in neural stem cells. By knocking out MBD3, an essential scaffold subunit of the NuRD complex, at different time points in reprogrammi...

2016
Tomas Barta Lucie Peskova Joseph Collin David Montaner Irina Neganova Lyle Armstrong Majlinda Lako

MicroRNA (miRNAs) are short noncoding RNA molecules involved in many cellular processes and shown to play a key role in somatic cell induced reprogramming. We performed an array based screening to identify candidates that are differentially expressed between dermal skin fibroblasts (DFs) and induced pluripotent stem cells (iPSCs). We focused our investigations on miR-145 and showed that this ca...

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