Sequencing of 16S rDNA, and of sodAint and rpoBint in some cases, was applied to DNA from heart valves of 46 patients (36 with definite and 10 with possible endocarditis). Sequence-based identifications were compared with those obtained with conventional methods. Among the 36 definite cases, 30 had positive blood cultures and 6 had negative cultures. Among the 30 positive cases, sequencing of 1...

Background: Bacterial mastitis in cows is a huge challenge to the dairy cattle breeding and production industry. This study examined relationship between bacterial diversity at different sampling sites farms. Methods: High-throughput sequencing of 16S rRNA V4 region was used analyze abundance population sampled from seven farm, named healthy cow milk (N), (C), perimammary (T), collecting cup (B...

Among the marine planktonic diatoms, Chaetoceros is among most species-rich genera, and many species are considered important primary producers. However, little known about ecology distribution of few small solitary within this genus, including tenuissimus. This article describes a minute strain, identified as C. tenuissimus (named CT16ED) that was isolated at coastal lagoon in Corsica Island, ...

AIM Rapid detection of H.pylori strains by PCR-Sequencing. METHODS 16S rDNA amplification by PCR from template genomic DNA, confirmation of amplicon size by agarose gel electrophoresis, sequencing of amplicons by automated sequencer, analysis of sequences by NCBI -BLAST software. RESULTS The PCR -Sequencing and analysis of the sequence data by BLAST resulted in detection of the strain to be...

Ribosomal DNA (rDNA) plays a key role in ribosome biogenesis, encoding genes for the structural RNA components of this important cellular organelle. These genes are vital for efficient functioning of the cellular protein synthesis machinery and as such are highly conserved and normally present in high copy numbers. In the baker's yeast Saccharomyces cerevisiae, there are more than 100 rDNA repe...

DNA breakage arises during a variety of biological processes, including transcription, replication and genome rearrangements. In the context of disease, extensive fragmentation of DNA has been described in cancer cells and during early stages of neurodegeneration (Stephens et al., 2011 Stephens et al. (2011) [5]; Blondet et al., 2001 Blondet et al. (2001) [1]). Stults et al. (2009) Stults et al...