PROCEDURES The following have been used with success: haematoxylin and eosin, Perls's reaction for ferric iron, the periodic acid-Schiff reaction for carbohydrates, the alcian blue method for acid mucopolysaccharides, the Feulgen reaction for desoxyribonucleic acid, the periodic acid-hexamine silver method, Luxol fast blue for myelin, solochrome cyanine as a general stain, Heidenhain's iron hae...

Study of the stoichiometry of the DNA-methyl green reaction by dialysis, precipitation of stain-nucleic acid mixtures, and the staining of nuclei of known DNA content, indicate that the compound consists of one dye molecule per 10 P. The significance of this result was discussed in the preceding paper (1). Histone and lanthanum (and probably other multivalent cations (3)) compete with the dye f...

Critical protein-protein interactions are ubiquitous in biology. To provide a new method to detect these interactions, we designed and synthesized fluorinated bromopyronins as molecular probes. These electrophilic compounds rapidly react with amines via a S(N)Ar mechanism to form modestly electrophilic aminopyronin fluorophores. To investigate whether proteins modified with aminopyronins might ...

An understanding of mechanisms regulating hematopoietic stem cell engraftment is of pivotal importance to the clinical use of cultured and genetically modified transplants. Human cord blood (CB) cells with lymphomyeloid repopulating activity in NOD/SCID mice were recently shown to undergo multiple self-renewal divisions within 6 days in serum-free cultures containing Flt3-ligand, Steel factor, ...

The identification and specific localization of chemical constituents within the cell nucleus have lagged considerably behind the qualitative and quantitative characterization of these classes of substances, with the possible exception of one component, the deoxyribonucleic acid (DNA). In addition to the latter, the nucleus contains ribonucleic acid1 2 (RNA), histone or basic protamine3 (BP), a...

The basiphil cell granules of the human anterior pituitary react positively with Gram's stain, but no specific differentiation of these granules occurs when the iodine treatment is omitted. The granules of the acidophil cells are Gram-negative. Various workers have suggested a connexion between the Gram reaction of various micro-organisms and the presence of ribonucleoprotein, and the pituitary...

Layer charge is one of the key parameters used for the characterisation of expandable clay minerals, smectites. It determines most significant properties of the material which are important from the industrial application point of view. This work is related to a novel method introduced to characterize the layer charge of smectites, based on using cationic organic dyes as molecular sensors. One ...

Correspondence: A. Martín-Noya, M.D., Servicio de Hematología, Hospital Universitario Virgen del Rocío, Avda. Manuel Siurot s/n1, 41013 Seville, Spain. Phone: international +34-95-4248122 – Fax: international +34-954248124 – E-mail: [email protected] A71-year-old woman was admitted to hospital because of repeated episodes of epistaxis and an eight month history of fatigue and weight loss. On ph...

OBJECTIVES The oxazolidinone class of antibiotics such as linezolid have a narrow spectrum of activity that targets Gram-positive bacteria. We hypothesized that the poor activity of linezolid in Gram-negative bacteria is in part caused by relatively low intracellular concentration due to efflux. METHODS Using whole cell accumulation assays we estimated the intracellular concentration of linez...

OBJECTIVES The emergence of antibiotic resistance has seriously diminished antibiotic efficacy and an increasing number of infections are becoming difficult to treat. One approach to the restoration of antibiotic activity is to administer them in conjunction with non-antibiotic compounds that depress resistance mechanisms. We describe the activity of ellagic and tannic acids as adjuvants that e...