Weimberg, Ralph (Northern Regional Research Laboratory, Peoria, Ill.), and William L. Orton. Synthesis and breakdown of the polyphosphate fraction and acid phosphomonoesterase of Saccharomyces mellis and their locations in the cell. J. Bacteriol. 89:740-747. 1965.-The conditions for accumulation of polyphosphate in cells of Saccharomyces mellis differ in several respects from those for acid pho...

The electric field method of gene transfer via electroporation of cell membranes is gaining importance in cellular and molecular biology. In particular the electroporation technique provides the low copy number transfection required fqr genetic studies. As demonstrated with mouse lyoma cells, with lymphoid cell lines and with plant protoplast, the electric method has appreciable advantages comp...

Analysis of oxidase and dehydrogenase activities, cytochrome content of mesosomal vesicles, and protoplast membranes showed that the respiratory chain in Staphylococcus aureus is associated predominantly with the protoplast membranes.

Abstract. For the first time, we present a cytomorphological description of self-healing (repair) process damaged thalli sites, wound healing , in green filamentous algae, Ulothrix zonata (F. Weber & Mohr) K tzing 1833. In filaments this species from Lake Baikal and Angara (Baikal outflow), Zhilishche inflow) Ida (Angara rivers, light microscopy methods revealed dome-like conical protrusion...

Departme Tusa, N., J.W. Grosser, and F.G. Gmitter, Jr. 1990. Plant regeneration of ‘Valencia’ sweet orange, ‘Femminello’ lemon, and the interspecific somatic hybrid following protoplast fusion. J. Amer. Soc. Hort. Sci. 115:1043– 1046. Vardi, A., P. Arzee-Gonen, A. Frydman-Shani, and S. Bleichman. 1989. Protoplast fusion mediated transfer of organelles from Microcitrus and Citrus and regeneratio...

Twenty-eight types of lysozyme-sensitive spores among seven Bacillus species representative of thermophiles, mesophiles, and psychrophiles were obtained spanning a 3,000-fold range in moist-heat resistance. The resistance within species was altered by demineralization of the native spores to protonated spores and remineralization of the protonated spores to calcified spores and by thermal adapt...

BACKGROUND Populus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function. METHODOLOGY/PRINCIPAL FINDINGS We optimized a Populus leaf mesophyll protoplast isolation protocol and established a Populus protoplast transient expression system. We demonstrated that Populus protoplasts ...

The formation of protoplast chains in suspensions of isolated pea (Pisum sativum cv Ran 1) mesophyll protoplasts induced by electric fields was studied. The chain formation induced by a sine-wave field (2 V, peak to peak; 500-0.1 kHz) was compared to that induced by an alternating pulse field (1 V, amplitude; 0.1-0.4 kHz). An increased number of dielectrophoretically paired protoplasts, formati...