Establishment and maintenance of pregnancy require the activity of a highly specialized maternal tissue, the decidua. It is well established that the human decidua synthesizes and releases prolactin. However, in the rat, no study has been able to demonstrate the production of prolactin by the decidua. In this report, we established for the first time using Northern blot analysis and reverse tra...

In mammary epithelial cells (MECs), prolactin-induced signaling and gene expression requires integrin-mediated cell adhesion to basement membrane (BM). In the absence of proper cell-BM interactions, for example, culturing cells on collagen-coated plastic dishes, signal propagation is substantially impaired. Here we demonstrate that the RhoA-Rok-myosin II pathway accounts for the ineffectiveness...

Background: This study investigated the effects of estradiol on plasma calcium and prolactin cells of Heteropneustes fossilis kept in calcium-deficient and normal freshwater. Methods: Fish were deprived of food and divided into groups A-D. Group A and B were kept in artificial freshwater with normal electrolytes. Group C and D were maintained in calcium-deficient freshwater. Vehicle was admin...

Prolactin iodinated by lactoperoxidase method showed immunologically, electrophoretically and biolo9gically similar properties to native prolactin and possessed enough specific radioactivity for receptor studies. 1251-prolactin was incubated with mouse mammary tissues at 8 days of lactation. Both binding and release of 1251-prolactin depended on incubation time and temperature and were maximal ...

14 Since anterior pituitary expresses prolactin receptors, prolactin secreted by lactotropes could 15 exert autocrine or paracrine actions on anterior pituitary cells. In fact, it has been observed that 16 prolactin inhibits its own expression by lactotropes. Our hypothesis is that prolactin participates 17 in the control of anterior pituitary cell turnover. In the present study we explored the...

a total of 125 russian red pied cows were genotyped for the prolactim-related gene. the prl-rsai genotypes were analysed using the polymerase chain reaction -restriction fragment length polymorphism (pcr-rflp) method. in this breed, the frequencies of alleles were as follows;  a= 0.794 and b= 0.206. the frequencies of aa, ab and bb genotypes were 0.598, 0.392 and 0.01; respectively. results sho...

Prolactin is best known as the polypeptide anterior pituitary hormone, which regulates the development of the mammary gland. However, it became clear over the last decade that prolactin contributes to a broad range of pathologies, including breast cancer. Prolactin is also involved in angiogenesis via the release of pro-angiogenic factors by leukocytes and epithelial cells. However, whether pro...

1) The evidence for a role of prolactin in the premenstrual syndrome is discussed in this review. (2) The timing of the onset and offset of both physical and psychological dysphoric symptoms corresponds with the luteal elevation and menstrual decrease of serum prolactin levels. (3) Women with premenstrual symptoms have been shown to have high prolactin levels throughout the menstrual cycle and ...

Since anterior pituitary expresses prolactin receptors, prolactin secreted by lactotropes could exert autocrine or paracrine actions on anterior pituitary cells. In fact, it has been observed that prolactin inhibits its own expression by lactotropes. Our hypothesis is that prolactin participates in the control of anterior pituitary cell turnover. In the present study, we explored the action of ...

Pituitary prolactin has been quantified by radioimmunoassay in whole milk obtained from cattle, goats, sheep and rats. Prolactin concentrations in milk samples obtained following the completion of lactogenesis approximate concentrations of the hormone in blood plasma or serum. However, concentrations of prolactin in prepartum mammary secretions were much higher than plasma prolactin in prepartu...