Conditioned media from single mouse ovarian follicles cultured from the early preantral stage up to complete maturity were analysed for different immunoreactive inhibin forms. The inhibin assays measured (i) alpha-specific inhibin, as represented by a mix of 32 and and 57 kDa inhibins, inhibin precursors, alpha-subunit and its precursors; (ii) dimeric inhibin A; and (iii) dimeric inhibin B. The...

Background: Cryopreservation of oocytes is an effective technology in assisted reproductive technology. Although successful vitrification of gametes has been reported in several mammalian species, the survival is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) on preantral follicle development after vitrifi...

The assembly of the primordial follicles early in ovarian development and the subsequent development and transition of the primordial follicle to the primary follicle are critical processes in ovarian biology. These processes directly affect the number of oocytes available to a female throughout her reproductive life. Once the pool of primordial follicles is depleted a series of physiological c...

OBJECTIVE Improvements in cancer treatment have allowed more young women to survive. However, many cancer patients suffer from ovarian failure. Cryopreservation is one of the solutions for fertility restoration in these patients. The cryopreservation of isolated follicles is a more attractive approach in the long term. Many endocrine and paracrine factors can stimulate the granulosa cells of pr...

objective: the purpose of the present study was to verify the ultrastructural characteristics of cultured mouse preantral follicles from fresh and vitrified –warmed ovarian tissues. materials and methods: the ovaries of 14-day-old mice (n = 5) were vitrified using a mixture of ethylene glycol, ficoll 70 and sucrose. the preantral follicles were isolated and cultured in α-minimum essential mediu...

In vitro follicle growth (IVFG) strategy is critical in the fertility preservation of cancer survivors; however, its optimal protocol needs to be developed using primate models since the availability of human samples is limited. Only a few previous studies have reported the successful IVFG of rhesus monkey ovaries using low-dose follicle-stimulating hormone (FSH) (0.3 or 3 ng/mL) and long-term ...

Lima-Verde I.B., Matos M.H.T., Celestino J.J.H., Rossetto R., Name K.P.O., Báo S.N., Campello C.C. & Figueiredo J.R. 2012. Progesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro. Pesquisa Veterinária Brasileira 32(4):361-367. Programa de Pós-Graduação em Ciências Veterinárias, Laboratório de Manipulação de Oócitos e Folículo...

To date, methods for quantifying cellular proliferation within the human ovary have relied primarily on static measurements at time of tissue recovery, such as labeling ki67 and phosphohistone-H3. Here, we describe a method based consecutive injection two thymidine analogues, ethynyl deoxyuridine (EdU) 5-chloro-2′-deoxyuridine (CldU), in ovarian xenograft model, allowing resolution division dis...

This study aims to investigate the effects of follicle stimulating hormone (FSH) and fibroblast growth factor-2 (FGF-2) on the survival and growth of caprine preantral follicles. Ovarian tissues were cultured for 1, 7, 14, 21 or 28 days in medium supplemented with FSH (FSH-2d or FSH-7d, i.e., with replacement of the culture medium every 2 or 7 days, respectively) or FSH+FGF-2 (replacement of th...

background: the ultrastructural analysis of cultured follicles could direct us to understand subcellular changes during in vitro culture. objective: this study was done to verify the ultrastructural characteristics of in vitro cultured mouse isolated preantral follicles in co-culture system in the presence and absence of leukemia inhibitory factor (lif) materials and methods: mechanically isola...