an agrobacterium-mediated transient gene expression assay was carried out in alfalfa (medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of foot and mouth disease virus called vp1. the plant leaves were transformed via agroinfiltration procedure. the presence of the foreign gene and its expression in transformed plants were evaluated by polymerase chain ...

This study aimed to compare the occurrence and level of Bacillus cereus sensu stricto (B. cereus) in chili, previously isolated white pepper from traditional supermarkets around Bogor, determine phylogenetic relationship between obtained isolates based on their 16S rDNA gene, potential toxicity ces nheA genes using Polymerase Chain Reaction. The highest presumptive B. samples was 5.95×105 2.6×1...

Type 2 diabetes mellitus (T2DM) is characterized by insulin resistance and insulin secretory defect. Deficiency of cellular immunity is known as one of the factors involved in the pathogenesis of T2DM. lymphocyte-specific protein tyrosine kinase( LCK) is an important gene involved in the intracellular signaling pathways of lymphocytes. This study aimed at determining and comparing LCK gene expr...

the aim of this study was to determine the frequency of enterotoxigenic and enteroaggregative strains ofescherichia coli in chicken carcasses by polymerase chain reaction (pcr). in this study 63 strains of e. coliwere isolated from 110 samples of chicken carcasses during processing after chilling in the poultry slaughterhouse of shahrekord. polymerase chain reaction assays were used to detect t...

We have compared the effect of the polyomavirus cis-acting transcriptional enhancer and the adenovirus trans-acting E1A gene on expression of RNA polymerase III-transcribed genes (the adenovirus VAI gene and a bacterial tRNA gene) using DNA transfection and transient expression assays. The polyomavirus enhancer has little effect upon transcription of the VAI gene by RNA polymerase III in any ce...

Beta catenin gene can inhibit the chondrogenic differentiation of human adipose tissue derived stem cells, while sex determining region Y-box9 promote cells. However, it is still necessary to study role combination two genes in early The purpose this was investigate whether knockdown and overexpression combined with modification cells could Therefore, divided into four groups or lentivirus vect...

Introduction: Burn infections are a serious obstacle to the patient’s recovery. Infection is estimated account for 75% of burn patient mortality. Widespread and often indiscriminate administration antibiotics lack basic infection control methods major factors in emergence drug-resistant bacteria. Due growing threat antibiotic resistance, interest silver compounds has grown modern medicine. Obje...

Abstract Twenty (25%) isolates of Proteus Mirabilis were isolated from a total 80 samples patients suffering otitis media and urinary tract infections. The collected various hospitals in Al-Diwaniyah city between December 2021 March 2022. was identified using cultural biochemical tests. diagnosis confirmed by polymerase chain reaction (PCR) to investigate the 16s rRNA gene, DNA sequencing regis...

Introduction: The TP53 and CHEK2 genes have been described as breast cancer susceptibility some of their polymorphisms associated with an increased risk in certain populations.Aim: objective this study was to investigate the p.R72P PIN3 Ins16bp (TP53) I157T (CHEK2) mutation developping cancer. Methods: This case-control had enrolled 144 participants including 65 cases (breast patients) 79 contr...

conclusions the results of this study demonstrated that the tpasp-padre-truncated orf2 gene cassette and the truncated orf2 gene in recombinant plasmids are successfully expressed in eukaryotic cells. the immunogenicity of the two recombinant plasmids with different formulations will be evaluated as a novel dna vaccine in future investigations. results sequence analysis and bioinformatics studi...