Nod2 is a cytosolic pattern recognition receptor. It has been implicated in many inflammatory conditions. Its signaling has been suggested to modulate TLR responses in a variety of ways, yet little is known about the mechanistic details of the process. We show in this study that Nod2 knockdown mouse peritoneal macrophages secrete more IL1β than normal macrophages when stimulated with peptidogly...

Innate immune responses to bacteria require cooperative interactions between host recognition molecules and phagocytes. The peptidoglycan recognition proteins (PGRPs) are a large group of proteins found in insects and mammals that bind to bacterial peptidoglycan (PGN). PGRP-S is located with other antimicrobial proteins, such as lysozyme, in the granules of human neutrophils. Whereas both PGRP-...

Let F be the field of real or complex numbers. For n ≥ 1, the subspaces of the n-dimensional inner product space F form a modular ortholattice PGn−1(F), or simply PGn−1. This lattice has a normalized dimension function dn : PGn−1 → [0, 1] that associates to a subspace A, its dimension divided by n. von Neumann [7] showed there is an embedding PGn−1 ↪→ PG2n−1 that preserves normalized dimensions...

Naturally occurring intestinal nanomineral particles constituently form in the mammalian gut and trap luminal protein and microbial components. These cargo loaded nanominerals are actively scavenged by M cells of intestinal immune follicles, such as Peyer's patches and are passed to antigen-presenting cells. Using peripheral blood mononuclear cell populations as an in vitro model of nanomineral...

The identification of an agent effective for the treatment of intestinal and bone marrow injury following radiation exposure remains a major issue in radiological medicine. In this study, we evaluated the therapeutic impact of single agent or combination treatments with 2-(3-aminopropylamino) ethylsulphanyl phosphonic acid (WR-2721) and peptidoglycan (PGN, a toll-like receptor 2 (TLR-2) agonist...

CARD15 is a major susceptibility gene for a frequent multifactorial chronic inflammatory bowel disorder, Crohn disease (CD). By using NF-kappaB activation assays, the cytosolic CARD15 was shown to efficiently detect bacterial peptidoglycan (PGN), reminiscent of the PGN recognition protein surveillance mechanism in Drosophila. The 3 CD-associated variants and 13 additional variants carried by CD...

Bacteriolyses of bacterial cell walls by zinc (II) ions on the basis of the results of halo antibacterial susceptibility tests were investigated for the nitrate and the sulfate solutions. From the results obtained by halo antibacterial tests of sulfate solutions against Staphylococcus epidermidis, the antibacterial order is Zn2+>Cu2+>Ag+>Al3+, in which Zn2+ ions indicate the highest antibacteri...

Heme oxygenase (HO)-1 is a cytoprotective enzyme with anti-inflammatory properties. HO-1 is induced during a systemic inflammatory response, and expression of HO-1 is beneficial during sepsis of a Gram-positive source. Systemic infection from Gram-positive organisms has emerged as an important cause of sepsis, with Staphylococcus aureus as a common etiology. An important mediator of Gram-positi...

Hussain T, Nasreen N, Lai Y, Bellew BF, Antony VB, Mohammed KA. Innate immune responses in murine pleural mesothelial cells: Toll-like receptor-2 dependent induction of -defensin-2 by staphylococcal peptidoglycan. Am J Physiol Lung Cell Mol Physiol 295: L461–L470, 2008. First published July 11, 2008; doi:10.1152/ajplung.00276.2007.—The innate immune response is mediated in part by pattern recog...

Recognition of microbial patterns by host pattern recognition receptors is a key step in immune activation in multicellular eukaryotes. Peptidoglycans (PGNs) are major components of bacterial cell walls that possess immunity-stimulating activities in metazoans and plants. Here we show that PGN sensing and immunity to bacterial infection in Arabidopsis thaliana requires three lysin-motif (LysM) ...