BACKGROUND & OBJECTIVES Infections due to seafood associated Salmonella serovars are great risk to public health. Different phenotypic characteristics have been used previously for epidemiological investigation of Salmonella. Beyond the phenotypic characterization, a reliable genetic level discriminatory method is required. Therefore, this study was attempted to use different phenotypic and mol...

Many reports highlight biological responses of crop plants after non-ionizing electromagnetic radiation (EMR) exposure based on the phenotypic and physiological levels. So, this study aimed to estimate genetic alterations in proteins, isozymes, DNA banding patterns as well extent nuclear damage economic corn (Zea mays) under stress EMR using accurate reliable bioassays like sodium dodecyl sulfa...

To elucidate the genetic diversity of ten Ipomoea species, seed proteins were analyzed by SDS-PAGE and RAPD analysis. According to SDS-PAGE analysis, in total 51 different bands were identified across the studied species. The number of bands varies from 16 bands in Ipomoea cairica, to 3 bands in I. sinensis. The similarity analysis based on the SDS-PAGE profile turned out to be a useful charact...

Abbreviations: CAPS: N-cyclohexyl-3-aminopropanesulfonic acid cDNA: omplementary DNA to mRNA CHES: N-cyclohexyl-2-aminoethanesulfonic acid. DTT: dithiothreitol FPLC: fast protein liquid chromatography IPTG: isopropylthio-β-D-galactoside LB: Luria-Bertani MES: 2-(N-morpholino)ethanesulfonic acid Ni-NTA: Nickel-nitriloacetic acid ORF: open reading frame PCR: polymerase chain reaction PMSF: phenyl...

The L4 molecular chirality can be amplified to a supramolecular scale by coassembling with SDS. Further incorporation of the CB[8] leads inversion via change from lamellar structure L4/SDS rectangular stacking in L4/SDS/CB[8].

1. Abstract 2. Intdocution 3. Methods 3.1. Materials 3.2. Isolation and culture of decidual cells 3.3. Digestion and purification of constructs 3.4. Transfection 3.5. Qauntitative PCR 3.6. SDS-Polyacrylamide Gel Electrophoresis and Western Blotting 3.7. β-gal staining 3.8. Statistical analysis 4. Results and discussion 4.1. Generation of Lefty-A tet on constructs 4.2. Construction and preparati...

The present study was conducted to observe the genotoxic effect of latex of Calotropis procera. Dense conidial suspensions of Aspergillus terreus Thorn were treated with different concentrations of Calotropis latex and assayed for percentage survival, auxotrophic mutants, DNA and total protein contents and further subjected to RAPD and SDS-PAGE tests. It was found that latex of Calotropis proce...