Back Cover: In article number 2100121, Suchetan Pal and co-workers develop a family of multi-responsive polymer hydrogels crosslinked with bis boronic acid molecules. Hydrogels tetrahydroxydiboron show glucoseresponsive degradation in the presence glucose oxidase (GOX) enzyme. Furthermore, insulin releases under high conditions physiological milieu.

In order to investigate physiological and biochemical changes of wheat under stress residue of Imazethapyr herbicide (Imazethapyr), an experiment was conducted in a completely randomized design with four replications in greenhouse conditions at university of Mohaghegh Ardebili. The treatments consisted of five dose levels of herbicide Imazethapyr (0, 0.011, 0.022, 0.033 and 0.044 micrograms of ...

The free radical-generating enzyme xanthine oxidase has been hypothesized to be a central mechanism of the injury which occurs in postischemic tissues; however, its importance remains controversial. Much attention has focused on the role of this enzyme in myocardial reperfusion injury. While xanthine oxidase has been observed in ischemic tissue homogenates, the presence and importance of radica...

Hexose oxidase from Chondrus crispus catalyzes the oxidation of a variety of mono- and disaccharides including D-glucose, D-galactose, maltose, and lactose. The enzyme has previously been partially purified and was reported to be a highly glycosylated, copper-containing protein with a relative molecular mass of approximately 130,000 (Sullivan, J. D., and Ikawa, M. (1973) Biochim. Biophys. Acta ...

The thermostabilization of some enzymes by amidination with dimethylsuberimidate was surveyed. In the enzymes investigated, a moderate increase of thermostability in glucose oxidase and invertase, and prominent increase in lactate dehydrogenase, alcohol dehydrogenase and ascorbate oxidase were observed. The increase of thermostability by amidination was suggesting the synergism of the enhanceme...

We examined the subcellular localizations of NAD(P)H oxidase, a reactive oxygen species (ROS)-producing enzyme, in fetal membrane chorion laeve trophoblasts from preterm or term pregnant women with or without chorioamnionitis (CAM). Ultrastructural enzyme histochemistry for NAD(P)H oxidase was used. In fetal membranes without CAM, approximately one quarter of the chorion laeve trophoblasts (25....

A novel enzyme, pyrroloquinoline quinone (PQQ)-dependent polyvinyl alcohol (PVA) dehydrogenase, was found in and partially purified from the membrane fraction of a PVA-degrading symbiont, Pseudomonas sp. strain VM15C. The enzyme required PQQ for PVA dehydrogenation with phenazine methosulfate, phenazine ethosulfate, and 2,6-dichlorophenolindophenol as electron acceptors and did not show PVA oxi...

Urate oxidase is an important enzyme with therapeutic and diagnostic applications. Rasburicase is a recombinant urate oxidase enzyme approved by FDA to use in the treatment of hyperuricemia conditions. Various hosts such as Saccharomyces cerevisiae, Hansenula polymorpha and Escherichia coli have been used to express the enzyme. Today, Pichia pastoris is considered as an important host for heter...

To assay the in vitro xanthine oxidase and Tyrosinase inhibitory activity of the alkaloid fractions of the leaves of Indigofera spalathoides and to determine its enzyme inhibition mechanism. Xanthine oxidase and Tyrosinase inhibitory activity was assayed spectrophotometrically under aerobic conditions and the degree of enzyme inhibition was determined by measuring the increase in absorbance at ...

Indoleacetic acid (IAA) oxidase has been reported to be involved in plant growth because of its alleged role in the control of endogenous IAA levels. This purported role was reevaluated in terms of the properties and subcellular location of the enzyme in etiolated pea (Pisum sativum L. var. Alaska) epicotyls.The enzymic properties of IAA oxidase in the floating pea epicotyl segments are similar...