The cytotoxic effects of ferric nitrilotriacetate (Fe-NTA) have been considered to be caused by free radicals produced by the drug. The present study was carried out to determine whether or not cytotoxic effects of Fe-NTA on cell growth and lipoperoxide formation of Chinese hamster cells were reduced by antioxidants. Using a spin trapping technique, we found that hydroxyl radical formation in t...

In this paper, we report on the functionalization of silicon nanostructured (NanoSi) surface with an organic layer of nitrilotriacetic acid (NTA) and its subsequent use as an affinity surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) interface for histidine-tagged peptide enrichment and mass spectrometry analysis. The NTA terminal groups are immobilized onto the NanoSi s...

UNLABELLED To develop a (99m)Tc renal tracer with a capacity to measure effective renal plasma flow comparable to that of the clinical gold standard (131)I-o-iodohippurate ((131)I-OIH) and superior to that of (99m)TcO-mercaptoacetyltriglycine, which has a clearance only 50%-60% that of (131)I-OIH, we investigated (99m)Tc-tricarbonyl nitrilotriacetic acid (Na(2)[(99m)Tc(CO)(3)(NTA)]). This radio...

This study describes a fast and accurate method for the sample preparation, identification, and quantitation of nitrilotriacetic (NTA) acid in environmental aqueous samples at a concentration of ppb level. The method is sensitive, specific, and free from the interferences of fatty and amino acids. The tri-n-propyl- and tri-n-butyl-NTA acid esters were prepared by the reaction of n-propyl-HCl an...

Eukaryotic initiation factor 5A (eIF-5A) is the only cellular protein known to contain a hypusine residue that is formed by transferring the aminobutyl moiety from spermidine to a specific lysine residue, followed by hydroxylation at the aminobutyl group. A simple PCR-based strategy was developed to obtain a full-length cDNA of Neurospora crassa eIF-5A. The strategy consists of (i) the design o...

To develop a 99mTc renal tracer with a capacity to measure effective renal plasma flow comparable to that of the clinical gold standard 131I-o-iodohippurate (131I-OIH) and superior to that of 99mTcO-mercaptoacetyltriglycine, which has a clearance only 50%260% that of 131I-OIH, we investigated 99mTc-tricarbonyl nitrilotriacetic acid (Na2[Tc(CO)3(NTA)]). This radiopharmaceutical, which is based o...

The mineralization of 1.0 to 100 ng each of four complexing compounds-oxalate, citrate, nitrilotriacetate (NTA), and EDTA-per ml was tested in media prepared in accordance with equilibrium calculations by a computer program so that the H, Ca, Mg, Fe, or Al complex (chemical species) was predominant. Sewage microorganisms mineralized calcium citrate more rapidly than iron, aluminum, or hydrogen ...

Small molecule-based fluorescent probes have been used for real-time visualization of live cells and tracking of various cellular events with minimal perturbation on the cells being investigated. Given the wide utility of the (histidine)6-Ni(2+)-nitrilotriacetate (Ni-NTA) system in protein purification, there is significant interest in fluorescent Ni(2+)-NTA-based probes. Unfortunately, previou...

In this paper we give a symbolic concurrent semantics for network of timed automata (NTA) in terms of extended symbolic nets. Extended symbolic nets are standard occurrence nets extended with read arcs and symbolic constraints on places and transitions. We prove that there is a complete finite prefix for any NTA that contains at least the information of the simulation graph of the NTA but keep ...