Exosomes are cell-derived vesicles and are abundant in biological fluids; they contain RNA molecules which may serve as potential diagnostic biomarkers in 'precision medicine'. To promote the clinical application of exosomal RNA (exoRNA), many isolation methods must be compared and validated. Exosomes in cell culture medium (CCM) and serum may be isolated using ultracentrifugation (UC), ExoQuic...

Btana is traditional preservation method that can sustain date’s supply during many years in comparaison to the commercial storage methods. However no scientific informations are available about biological factors that contribute to the successful of this method. Bacterial communities are a major key in preservation of many foods. Culture independent techniques are the most powerful tools to en...

The CRE1/AHK4 cytokinin receptor is an important component of plants' hormone signaling systems, and compounds that can alter its activity have potential utility for studying the receptor's functions and/or developing new plant growth regulators. A high throughput method was developed for screening compounds with agonist or antagonist properties toward the CRE1/AHK4 cytokinin receptor in a sing...

BACKGROUND Endovenous laser ablation (EVLA) is a treatment option for lower extremity varicose veins. In the present study, we investigate to the genetic changes and possibility of living tissue in the saphenous vein wall after the EVLA procedure. METHODS Eleven saphenous vein grafts were randomized in two groups: (1) 4 cm SVG segments of performed EVLA procedure in study group, (2) 4 cm segm...

48 RESULTS Sequence characterization of goat TLR genes 4.1. Isolation and quantification of RNA Total RNA was extracted from different tissues of goat and used for cDNA synthesis to amplify goat TLR4 and TLR8 genes for cloning and sequencing. The RNA extracted by TRIzol method was further purified using RNeasy MinElute Clean-up kit (Qiagen). The concentration and purity of RNA was measured by N...

Mangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The content is influenced by bacterial community that inhabits ecosystem, but information regarding community, especially mangrove root system, not widely available. There are several challenges completing this information, one of method used still a conventional form. Developm...

Background: Formalin-fixed paraffin-embedded (FFPE) specimen archives are a valuable source of sample material for molecular biological analysis. However, the DNA isolated from FFPE samples is usually low in concentration and fragmented. Thus, it necessary to optimize extraction protocol obtain best results. Proteinase K incubation undoubtedly crucial procedures, but this step often not well ex...

PURPOSE The study is aimed at finding new antibiotic therapy for aquaculture due to potential of bacteria to develop resistance to the existing therapies. Use of large quantities of synthetic antibiotics in aquaculture thus has the potential to be detrimental to fish health, to the environment and wildlife and to human health. METHODS Antimicrobial potential of volatile oil and fractions of c...

PURPOSE The extraction of nucleic acid is initially a limiting step for successful molecular-based diagnostic workup. This study aims to compare the effectiveness of three automated DNA extraction systems for clinical laboratory use. MATERIALS AND METHODS Venous blood samples from 22 healthy volunteers were analyzed using QIAamp Blood Mini Kit (Qiagen), MagNA Pure LC Nucleic Acid Isolation Ki...

Background: In eukaryotes, Histone acetylation and deacetylation plays a prominent role in controlling gene expression chromatin structure modifications. Both acetyltransferases (HAT) deacetylases (HDACs) work opposition to regulate acetylation. Reduced levels of histone H4 increased susceptibility the rice disease M. oryzae result from overexpression HDT701 rice. By changing defense-related pa...