Clarifying the identity and enzymatic activities of microorganisms associated with the decomposition of organic materials is expected to contribute to the evaluation and improvement of composting processes. In this study, we examined the cellulolytic and hemicellulolytic abilities of bacteria isolated from sawdust compost (SDC) and coffee residue compost (CRC). Cellulolytic bacteria were isolat...

Reporter gene systems are useful for studying bacterial molecular biology, including the regulation of gene expression and the histochemical analysis of protein products. Here, two genes, β-1,4-mannanase (manB) from Bacillus pumilus and β-glucuronidase (gusA) from Escherichia coli K12, were cloned into the expression vector pELX1. The expression patterns of these reporter genes in Lactobacillus...

The manA gene of Thermoanaerobacterium polysaccharolyticum was cloned in Escherichia coli. The open reading frame of manA is composed of 3,291 bases and codes for a preprotein of 1,097 amino acids with an estimated molecular mass of 119,627 Da. The start codon is preceded by a strong putative ribosome binding site (TAAGGCGGTG) and a putative -35 (TTCGC) and -10 (TAAAAT) promoter sequence. The M...

This study is concerned with the optimization of fermentation parameters for the hyper production of mannanase from Fusarium oxysporum SS-25 employing two step statistical strategy and kinetic characterization of crude enzyme preparation. The Plackett-Burman design used to screen out the important factors in the culture medium revealed 20% (w/w) wheat bran, 2% (w/w) each of potato peels, soyabe...

MANB36, a secrete endo-1,4-d-mannanase produced by Bacillus subtilis B36, was purified to homogeneity from a culture supernatant and characterized. The optimum pH value for the mannanase activity of MANB36 is 6.4 and the optimum temperature is 50 ∞C. The enzyme activity of MANB36 is remarkably thermostable at 60 ∞C and the specific activity of MANB36 is 927.84 U/mg. Metal cations (except Hg2+ a...

An a-D-galactosidase (EC 3.2.1.22) and a fl-D-mannanase (EC 3.2.1.78), which were secreted into the growth medium when Aspergillus tamarii was cultivated in the presence of galactomannan, were purified by a procedure including chromatography on hydroxyapatite and DEAE-cellulose columns. Each of these enzymes showed a single protein band, corresponding to their respective activities, on polyacry...

this experiment was carried out to evaluate the effect of different levels guar meal and β-mannanase on broilers male chicken. in this experiment , four hundred ross day old broiler chicks were randomly assigned to a 4×2 factorial design to feed broiler chickens diets containing guar meal at 4 level of guar meal (0, 3, 6 and 9%) with five replications of each treatment with or without β-mannana...

This study focused on the characterization of mannanase from Bacillus circulans NT 6.7 for mannooligosaccharides (MOS) production. The enzyme from B. circulans NT 6.7 was produced using defatted copra meal as a carbon source. The mannanase was purified by ultrafiltration and column chromatography of Q-Sepharose. The purified protein (M1) was a dimeric protein with a 40 kDa subunit. The purified...

Thirty-six species o f wood-inhabiting microfungi have been assayed for cellulase, xylanase, mannanase and amylase activity by the use o f different test methods. The wood-degrading capabilities of the test organisms were previously known. Three o f the species were unable t o degrade birch wood. The other species represented three different degradation patterns, viz. I ) formation of soft rot ...

An alkaliphilic Bacillus isolate (strain JAMB-750) was found to exoproduce a novel alkaline mannanase (AmA). AmA was purified to homogeneity (about 98% pure) as judged by SDS-polyacrylamide electrophoresis. The molecular mass was approximately 130 kDa. The hydrolysis patterns of various substrates indicated that the enzyme was an endo-type enzyme. The optimal temperature and pH for activity wer...