We investigate the immobilization of a model system of functionalized yeast that surface-display enhanced green fluorescent protein (eGFP) within chemically crosslinked polyvinyl alcohol (PVA) nanofibers. Yeast is incorporated into water insoluble nanofibrous materials by direct electrospinning with PVA followed by vapor phase chemical crosslinking of the polymer. Incorporation of yeast into th...

Several microscopy techniques are available today that can detect a specific protein within the cell. During the last decade live cell imaging using fluorochromes like Green Fluorescent Protein (GFP) directly attached to the protein of interest has become increasingly popular. Using GFP and similar fluorochromes the subcellular localisations and movements of proteins can be detected in a fluore...

Layout, operation, and results of a microfluidic device, which allows for anaerobic and aerobic cultivation of yeast cells over extended time (>3 days) are presented. The use of this device in combination with automated image analysis software and multiple fluorescence reporters (>4 fluorescence protein per strain) allows for studying timing and coherence of morphological features, the cell-cyc...

ong-range chromosome organization is known to influence nuclear function. Budding yeast centromeres cluster near the spindle pole body, whereas telomeres are grouped in five to eight perinuclear foci. Using live microscopy, we examine the relative positions of right and left telomeres of several yeast chromosomes. Integrated lac and tet operator arrays are visualized by their respective repress...

Thirty crossbred sows and their pigs were evaluated through two parities to determine any reproductive or growth performance effects of an active dry yeast supplement added to corn-soybean meal diets. Sow reproductive performance from d 93 of gestation through d 21 of lactation and sow milk composition were evaluated. Pig growth performance was measured from birth to 28 d after weaning. Active ...

The purposes of this study were to investigate the optimum UV-B irradiation time needed produce vitamin D enriched yeast and promote in vitro ruminal degradation irradiated Thai native cattle. Baker’s S. cerevisiae at 0, 2, 4, 8, 10, 12, 16 24 hours. both strains could significantly enhance D2 highest amount corn stover TMR studied for determination degradation, included three treatments that i...

Currently, killed-virus and modified-live porcine reproductive and respiratory syndrome virus (PRRSV) vaccines are used to control porcine reproductive and respiratory syndrome. However, both types of vaccines have inherent drawbacks; accordingly, the development of novel PRRSV vaccines is urgently needed. Previous studies have suggested that yeast possesses adjuvant activities, and it has been...

Kinetochore capture and transport by spindle microtubules plays a crucial role in high-fidelity chromosome segregation, although its detailed mechanism has remained elusive. It has been difficult to observe individual kinetochore-microtubule interactions because multiple kinetochores are captured by microtubules during a short period within a small space. We have developed a method to visualize...