Coating of liposomes with polyethylene-glycol (PEG) by incorporation in the liposome bilayer of PEG-derivatized lipids results in inhibition of liposome uptake by the reticulo-endothelial system and significant prolongation of liposome residence time in the blood stream. Parallel developments in drug loading technology have improved the efficiency and stability of drug entrapment in liposomes, ...

Unmethylated cytosine-phosphorothioate-guanine oligodeoxynucleotides (CpG-ODNs) exhibit potent immunostimulating activity by binding with Toll-like receptor 9 (TLR9) expressed on antigen-presenting cells. Here, we show that CpG-ODN encapsulated in cationic liposomes (CpG-liposomes) improves its incorporation into CD11c(+) dendritic cells (DCs) and induces enhanced serum interleukin (IL)-12 leve...

Directed evolution of proteins is a technique used to modify protein functions through "Darwinian selection." In vitro compartmentalization (IVC) is an in vitro gene screening system for directed evolution of proteins. IVC establishes the link between genetic information (genotype) and the protein translated from the information (phenotype), which is essential for all directed evolution methods...

Intraphagocytic (neutrophil and monocyte) concentrations of free and liposome encapsulated ampicillin were investigated in sheep. The ampicillin (5 mg/kg b.wt.) was administered as intravenous infusion. Blood samples were collected at 0, 10, 30, and 60 min, and 2 and 4 h after the infusion. Neutrophils and monocytes were isolated and lysed. Ampicillin concentrations were measured by high perfor...

Liposomes entrapping fluorescein diacetate were fused with protoplasts of Datura innoxia Mill by employing polyethylene glycol (PEG) as the fusogen. Factors that influence liposome-protoplast fusion were optimized as a function of PEG-concentration and incubation duration, liposome composition and surface charge and liposome:protoplast ratio. Phosphatidylcholine-liposomes were found ideal for t...

Oxidative stress can affect in vitro GFP expression through its control of the gene silencing effect of the liposome prepared by 1,2-dioleoyl-3-trimethyl-ammonium propane (DOTAP). The gene silencing effect of cationic DOTAP liposome in in vitro GFP expression, especially focusing on its translation process, and the effects of oxidative stress on its silencing effect were investigated. GFP expre...

At present, the effects of quercetin liposome on anxiolytic activity and cognitive function are unclearly known. In this study, male Wistar rats were administered quercetin liposome via intranasal route once daily continually for 4 weeks. The anxiolytic activity and cognitive enhancing effect were determined after single administration, 1, 2, 3 and 4 weeks of treatment using elevated plus maze ...

The effect of cholesterol on the molecular transport of an organic cation, malachite green (MG), across large unilamellar dioleolyphosphatidylglycerol (DOPG) liposome bilayers with 0-50 mol% cholesterol was studied by second harmonic generation (SHG). Because SHG is a surface-specific technique, it requires no labeled molecule, quencher, or shifting agent to distinguish the location of the solu...

combination formulations of liposomes with collagen have been previously introduced as a means of obtaining more stable and less permeable liposomes. in this study, the effect of aqueous solutions of the collagen products collapur? (col) and collapuron-dak? (cold) from henkle co., on the release rate of sodium chromate (chr) as a water-soluble model drug from stable plurilamellar vesicles (splv...