Lipase plays an important role in industrial and biotechnological applications. Lipases have been subject to modification at the N and C terminals, allowing better understanding of lipase stability and the discovery of novel properties. A thermotolerant lipase has been isolated from Antarctic Pseudomonas sp. The purified Antarctic AMS3 lipase (native) was found to be stable across a broad range...

Multifunctional enzymes have alternative functions or activities, known as “moonlighting” or “promiscuous”, which are often hidden behind a native enzyme activity and therefore only visible under special environmental conditions. In this thesis, the active-site of Pseudozyma (formerly Candida) antarctica lipase B was explored for a promiscuous conjugate addition activity. Pseudozyma antarctica ...

The oleaginous yeast Moniliella spathulata R25L270 was the first yeast able to grow and produce extracellular lipase using Macaúba (Acrocomia aculeate) cake as substrate. The novel lipase was recently identified, and presented promising features for biotechnological applications. The M. spathulata R25L270 lipase efficiently hydrolyzed vegetable and animal oils, and showed selectivity for genera...

Lipases (Triacylglycerol acylhydrolase) are industrially important enzymes that are mainly used in the food and pharmaceutical industry. Lipase enzymes are also used in food materials, detergent, medicine, cosmetics, leather, perfumery and other items. The enzyme was isolated from Myroides odoratimimus and optimized to different physiochemical characteristics, giving its optimum temperature 37°...

The use of enzymes in detergent formulations is becoming popular due to the concerns about the environment. T1 lipase (E.C. 3.1.1.3) was evaluated for its stability and performance in dishwashing along with other common components of an automatic dishwashing detergent. Therefore, the process of formulating the detergent would depend on the stability of T1 lipase, which may also reflect the perf...

In this essay, lipase from Burkholderia cepacia was immobilized into 3-glycidoxypropyltrimethoxysilane (GPTMS) and tetramethoxysilane (TMOS) derived sol-gels. GPTMS:TMOS molar ratio of 1:3 was found to yield the best result. The morphological characteristics were investigated based on SEM and BET analysis. Sample mean pore diameter was 39.1 nm, it had a specific surface area of 60 m2/g prior to...

Hydrocarbon synthesis from (waste)oils enabled by a cascade of lipase-catalysed hydrolysis and decarboxylase-catalysed decarboxylation has become an active area research en route to alternative, biobased fuels. However, Poor substrate transport efficiency is major issue causing low reaction rates. This study focused on protein self-assembly strategy based SpyTag/SpyCatcher overcome diffusion li...

Linoleic acid hydroperoxides are versatile intermediates for the production of green note aroma compounds and bifunctional ω-oxo-acids. An enzyme cascade consisting lipoxygenase, lipase catalase was developed one-pot synthesis 13-hydroperoxyoctadecadienoic starting from safflower oil. Reaction conditions were optimized hydroperoxidation using lipoxygenase 1 Glycine max (LOX-1) in a solvent-free...

A number of coagulase-negative staphylococci isolated from human skin were found to produce lipase. Lipolytic activity appeared in the growth medium during the stationary phase of growth but did not appear as a result of autolysis of the cells. Maximal lipase synthesis was obtained when the medium was adjusted to pH 7.5 before inoculation. The purified enzyme hydrolyzed tributyrin and tridecano...