Threonine deaminase (l-threonine dehydratase EC 4.2.1.16) has been partially purified from a new extreme thermophilic bacterium, Thermus X-1, which is similar to T. aquaticus YT-1. The threonine deaminase of strain X-1 has a maximal rate of reaction at 85 to 90 C and is more thermostable than the threonine deaminase from mesophilic bacteria. The enzyme has an apparent molecular weight of 100,00...

the objective of this study was to investigate the effects of three levels of threonine (100, 95 and 90% of nrc requirements) with or without of multi strains probiotic (primalac) on performance, carcass characteristics and blood metabolites of japanese quails. a total of 180 one-day-old male japanese quails were randomly allocated to six treatments with three replicates and 10 birds per each. ...

L-Threonine dehydrogenase, which catalyzes the conversion of L-threonine to aminoacetone + CO2 presumably via the intermediate formation of alpha-amino-beta-ketobutyrate, has been purified to apparent homogeneity from extracts of a mutant of Escherichia coli K-12 which has constitutively derepressed levels of the enzyme. Three fractionation steps were used including controlled heat denaturation...

This works reports on the linear and nonlinear optical properties of L-threonine crystals. Linear properties such as absorption spectra and dispersion of the refractive index are presented. Through second harmonic generation measurements we characterized the phase-matching loci for this sample, which presents conversion efficiency equivalent to that ofr KDP. The nonlinear refractive index n2, m...

Four chick bioassays were conducted to evaluate the threonine (Thr) replacement value of l-homoserine (HS). Growth rate was increased (P < 0.05) by dietary addition of 800 mg l-HS/kg diet to a purified diet severely deficient in Thr or by the addition of 800 or 1000 mg of l-HS/kg diet to a corn-peanut meal diet distinctly deficient in Thr. The addition of an isomolar level of alpha-ketobutyrate...

The micro-organism LC, isolated from the sheep rumen by Elsden, Volcani, Gilchrist & Lewis (1956), ferments a variety of substrates to yield volatile fatty acids (Elsden & Lewis, 1953). Washed cells of the organism fermented L-threonine and L-serine. Hydrogen, carbon dioxide and ammonia were produced from both substrates and, in addition, propionate was formed from threonine and acetate from se...

The enzyme L-threonine dehydrogenase catalyses the NAD(+)-dependent conversion of L-threonine to 2-amino-3-ketobutyrate, which is the first reaction of a two-step biochemical pathway involved in the metabolism of threonine to glycine. Here, the crystallization and preliminary crystallographic analysis of L-threonine dehydrogenase (Tk-TDH) from the hyperthermophilic organism Thermococcus kodakar...

this experiment was conducted to evaluate the effect of different levels of crude protein and threonine on performance, carcass characteristics and immune system of broiler chickens during starter (7-24) and grower (25-42) periods. a total of 288 male broiler chicks of ross 308 strain were used in a completely randomized design with factorial arrangement (2×4) with three replicates in each trea...

A psychrophilic bacterium, Cytophaga sp. strain KUC-1, that abundantly produces a NAD(+)-dependent L-threonine dehydrogenase was isolated from Antarctic seawater, and the enzyme was purified. The molecular weight of the enzyme was estimated to be 139,000, and that of the subunit was determined to be 35,000. The enzyme is a homotetramer. Atomic absorption analysis showed that the enzyme contains...

1. Measurements of the net synthesis of glucose plus glycogen from various precursors in slices of glycogen-depleted livers from rats at various stages of development indicated an increase in the gluconeogenic capacity after birth with l-lactate, oxaloacetate, a casein hydrolysate, l-serine, l-threonine, l-alanine and glycerol as substrates. 2. The highest rates of incorporation of (14)C-labell...