نتایج جستجو برای: isolation mitochondria

تعداد نتایج: 176965  

Journal: :The Journal of biological chemistry 1968
D V Cohn R Bawdon R R Newman J W Hamilton

When toxic doses of CC& were given to rats, there ensued marked accumulations of Ca++ and inorganic orthophosphate by whole liver commencing 6 hours after treatment. When these livers were homogenized in 0.25 M sucrose, the Ca++ and Pi concentrations of the mitochondria subsequently harvested were elevated several fold, reflecting the higher levels of these ions in the whole tissue. The Kf conc...

Background: Although the bio kinetics, metabolism and chemical toxicity of Atorvastatin are well known, until recently little attention was paid to the potential neurotoxic effect of Atorvastatin (Atv). Regarding the concrete evidences indicating Atv may reduce Coenzyme Q10 (CoQ10) levels through blockage of  metalonate cycle, the present work aims to determine if Atorvastatin may provide toxic...

Journal: :The Journal of Cell Biology 1967
Helen A. Mintz David H. Yawn Brian Safer Edward Bresnick Annabel G. Liebelt Zack R. Blailock Erwin R. Rabin Arnold Schwartz

A combined morphological and biochemical investigation of mitochondria from developing and rapidly growing tissues ( tumors, fetal, and very early neonatal rat liver) revealed mitochondria which were deficient in respiratory control, showed no valinomycin induced K(+) accumulation or spontaneous Ca(++) uptake, and were unable to undergo a swelling-contraction cycle. Electron microscopic examina...

Journal: :Plant physiology 1970
J K Raison J M Lyons

The respiration of mitochondria isolated from various plant tissues was studied over a range of mitochondrial concentrations and at various times after isolation. Respiration at 25 C expressed as nanomoles of O(2) per minute per milligram of protein was constant for mitochondrial concentrations higher than some critical amount, usually 0.25 to 1.0 milligram of protein per reaction. Below this c...

Journal: :The Biochemical journal 1975
N Collins M J Merrett

Isolation of organelles from broken-cell suspensions of phototrophically grown Euglena gracilis Klebs was achieved by isopycnic centrifugation on sucrose gradients. 2. Equilibrium densities of 1.23g/cm3 for peroxisome-like particles, 1.22g/cm3 for mitochondria and 1.17g/cm3 for chloroplasts were recorded. 3. The enzymes glycollate dehydrogenase, glutamate-glyoxylate aminotransferase, serineglyo...

Journal: :Plant physiology 1968
D D Killion S Grooms R E Frans

Mitochondria isolated from 2 strains of cotton plant hypocotyls (Gossypium hirsutum L. var. Rex smooth leaf and Rex glandless) were examined for their oxidative phosphorylation activities. Bovine serum albumin at a relatively high concentration was essential in the extraction medium for the isolation of oxidatively active mitochondria from both strains of cotton. Phosphorylation was obtained on...

Journal: :Journal of cell science 2002
Florian Fuchs Holger Prokisch Walter Neupert Benedikt Westermann

The establishment and maintenance of the 3D structure of eukaryotic cells depends on active transport and positioning of organelles along cytoskeletal elements. The biochemical basis of these processes is only poorly understood. We analysed the interaction of mitochondria with microtubules in the filamentous fungus Neurospora crassa. Mitochondria were fluorescently labelled by expression of mat...

2017
Evelina Rekuviene Laima Ivanoviene Vilmante Borutaite Ramune Morkuniene

The data presented in this article are related to the research article entitled "Rotenone decreases ischemia-induced injury by inhibiting mitochondrial permeability transition in mature brains" (Rekuviene et al., 2017) [1]. Data in this article present the direct effects of rotenone on calcium retention capacity (CRC) in isolated normal cortex and cerebellum mitochondria, effects of rotenone in...

2014
James D. McCully Janine M. Preble Hiroshi Kondo Sidney Levitsky

Transplant of mitochondria for cardioprotection has been shown to be efficacious in vitro and in vivo in animal models. The transition from animal to human models requires that effective quality control be used in the isolation and verification of mitochondrial viability and function. Herein, we describe standard procedures that can be used to isolate mitochondria and assess their quality and f...

Journal: :Journal of bacteriology 1967
M Federman C J Avers

Crosses were made between haploid wild-type and suppressive petite strains of bakers' yeast to obtain zygotes for analysis of mitochondrial heterogeneity. Wild-type x petite zygotes contained about 40% noncristate mitochondria when immediate mating mixtures were examined. The frequency of defective mitochondria had decreased to an average of 9.2% in 1-week-old zygote isolate cultures, and to 4....

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