The muscarinic receptor mediating vasodilation of resistance vessels in the rat isolated, constant-pressure perfused kidney (preconstriction by 10(-7) M cirazoline) was characterized by subtype-preferring agonists and selective antagonists. The agonists produced vasodilation with the following rank order of potency: arecaidine propargyl ester (APE) > 5-methylfurtrethonium = methacholine = oxotr...

Isolated tissue preparations are commonly used to study the effects of drugs on specific type of receptors. These preparations are also used for bioassay of drugs, characterisation of specific receptor or its subtypes, to determine concentration response curve of an agonist, to study antagonism of drug and in new drug discovery.[1] Isolated heart and rectus abdominis of frog are the two commonl...

Recent studies have shown that phloridzin, an inhibitor of sodium-glucose cotransporter (SGLT), strongly decreases high K+-induced contraction in phasic muscle, such as tenia coli, but slightly affects tonic muscle, such as trachea . In this study, we examined the inhibitory mechanism of phloridzin on high K+-induced muscle contraction in rat ileum, a phasic muscle. Phloridzin inhibited the hig...

seeds of moringa peregrina (forssk.) fiori. (moringaceae) is widely used in south east of iran for gastrointestinal disorders. however, so far there is no pharmacological evidence for antispasmodic activity of this plant extract. therefore, the aim of this research was to investigate antispasmodic activity of m. peregrina on rat isolated ileum contraction. hydroalcoholic extract was obtained by...

OBJECTIVE To investigate the action of N-acetylcysteine (NAC) on rat isolated ileal contractility, and to determine the effects of NAC on histopathological changes on ileal tissue. METHODS The study took place at the Faculty of Medicine, Ankara University, Ankara, Turkey, in January 2003. Adult Wistar rats were used in all experiments. Two groups were designed. The experimental group, to whic...

This study was aimed at evaluating the effect of Dihydroartemisinin (DHA)on contractility to drugs in ileum isolated smooth muscle preparations guinea pigs using Vitro experimental procedures / standard protocols an organ bath set up. dihydroartemisinin (1.0 x 10 - 8 1.0 – 5mg/mL) when applied alone and separately excited marked variable effects on pig . In some preparation it showed no respons...

Monospecific antibodies to the calcium transport enzyme (alpha-Ca pump) inhibit mitosis when microinjected into sand dollar embryos. Immunoglobulins were raised against the calcium transport enzyme (Ca pump) of sarcoplasmic reticulum (SR) from rat skeletal muscle and guinea pig ileum smooth muscle. Specific antibodies were further isolated from IgG fractions by using electrophoretically purifie...

Wood creosote has long been used as an antidiarrheal agent, but its mechanism of action is not well understood. To elucidate the mechanism of its antidiarrheal activity, we have addressed questions whether it inhibits fluid secretion induced by Escherichia coli heat-stable enterotoxin (STa) in rabbit jejunum in vivo, and whether it inhibits muscle contraction of isolated rabbit ileum ex vivo. W...

The mechanism of action of prostacyclin (PGI2) on isolated segments of guinea-pig terminal ileum was studied by recording the changes in isometric tension. In these preparations PGI2 (1 nM-1 microM) caused a concentration-dependent increase in muscle tension. This effect was rapid and short-lasting. PGI2-induced contractions were inhibited by atropine and potentiated by physostigmine. Hemicholi...