acetone fractionated peroxidase from crude extract of brassica oleracea leaves (cabbage) was purified in three steps on chromatographic columns, using sp-sepharose, deae-sepharose and con a-sepharose. the specific activity of purified main isoenzyme (boc-pod) is 1887 u/mg protein with rz: 3.1, which is 172 times more than the rz of crude extract with 4.3% recovery. the molecular weight of boc-p...

The X-linked enzyme, glucose-6-phosphate dehydrogenase (G-6-PD) was used as a cellular marker to study the clonal characteristics of human atherosclerotic lesions from females heterozygous for G-6-PD isoenzymes. Portions of uninvolved aortic wall contained both isoenzyme types (A and B), and their isoehzyme patterns were used to establish criteria for polyclonal lesions. Portions of uterine lei...

The organ distribution of rat histidine-pyruvate aminotransferase isoenzymes 1 and 2 was examined by using an isoelectric-focusing technique. Isoenzyme 1 (pI8.0) is present only in the liver and its activity is increased by the injection of glucagon, whereas isoenzyme 2 (pI5.2) is distributed in all tissues (liver, kidney, brain and heart) tested, and is not affected by glucagon injection. Isoe...

Total creatine kinase (CK) activity and isoenzyme distribution were determined in normal and tumour of gastrointestinal (GI) tract. Total CK activity per gram wet tissue appeared to be markedly reduced in malignant tumour tissue, yet it was raised in benign tumour tissue. CK-BB was the predominant isoenzyme in both normal and tumour tissue of GI tract. Alteration of isoenzyme pattern was noted ...

The effects of KS-505a, a novel microbial metabolite, on the activity of calmodulin-dependent cyclic nucleotide phosphodiesterase (CaM-PDE) were investigated. (1) KS-505a potently inhibited the purified 61 kDa isoenzyme of CaM-PDE from bovine brain and required much higher doses to inhibit the purified 59 kDa isoenzyme of CaM-PDE from bovine heart. The inhibition of both isoenzymes was observed...

in this study, we used random amplified polymorphic dna (rapd) for identification of 17 isolates of leishmania from the skin and reticuloendothelial system of humans, animal reservoirs (rodent and dog) and sandflies in various parts of iran in the last decade. fifteen species have been confirmed by isoenzyme characterization by the london school of hygiene and tropical medicine and shiraz unive...

It has been suggested-:" that examination of the serum lactic dehydrogenase (LDH) isoenzyme pattern may be helpful in identifying tissues affected by disease. Cellular damage to tissues such as myocardium or liver results in characteristic abnormalities in the relative proportions of the 5 LDH isoenzymes demonstrable in serum by electrophoresis in barbituratebuffered agar2•4, starch-grain! and ...

Intracellular lactate dehydrogenase (LD) isoenzyme patterns were studied in the malignant cells of patients with a variety of lymphoid malignancies. These were compared with intracellular LD isoenzyme patterns of normal lymphoid cells and were also correlated with immunologic cell surface marker characteristics. Results showed that, in general, the malignant B cells of Burkitt's lymphoma and th...

treatment with PLA,. At this stage it is impossible to say whether the presence of such large amounts of lysophospholipids has a role in isoenzyme inactivation. Nevertheless it can be hypothesized that each P450 isoenzyme responds differently to changes in membrane NEFA content and composition. This situation might facilitate the selective modulation of P450 isoenzyme activity depending on the ...