نتایج جستجو برای: ige binding proteins
تعداد نتایج: 865783 فیلتر نتایج به سال:
background one disadvantage of expressing heterologous proteins in escherichia coli is that the proteins are frequently expressed as insoluble inclusion bodies. to avoid this problem, heterologous proteins are typically expressed as a fusion protein. maltose binding protein (mbp) is one of the widely used partners for production of recombinant fusion proteins in e. coli. mbp is among the most e...
Peanut allergy is a significant IgE-mediated health problem because of the increased prevalence, potential severity, and chronicity of the reaction. Following our characterization of the two peanut allergens Ara h 1 and Ara h 2, we have isolated a cDNA clone encoding a third peanut allergen, Ara h 3. The deduced amino acid sequence of Ara h 3 shows homology to 11S seed-storage proteins. The rec...
Summary House dust mites are among the most important allergy triggers worldwide. While of genus Dermatophagoides occur almost worldwide, tropical mite Blomia tropicalis and storage only importance for certain areas or groups people. The allergens pteronyssinus Der p 1, 2, 23 with immunoglobulin E (IgE)-binding frequencies more than 70% high allergenic activity. Also 5, 7, 21, which have IgE-bi...
Background: Aspergillus species has crucial role in respiratory allergy. This study was aimed to evaluate the pattern of IgE-mediated immune response to common aspergillus species including Aspergillus fumigatus, Aspergillus flavous, Aspergillus niger in type I aspergillus-hypersensitive patients’ sera. Methods: The prepared protein extract of Aspergillus species were dialyzed and the ...
PURPOSE Eosinophils are frequently found in atopic dermatitis (AD) and chronic spontaneous urticaria (CSU) that release eosinophil peroxidase (EPX) cationic protein (ECP). Continuous exposure to these proteins could trigger an autoimmune response which may contribute the pathogenesis severity of skin inflammation. In this study, we investigate immunoglobulin E (IgE) against CSU AD. METHODS We r...
The thermal stability and IgE binding of raw and boiled shrimp extracts and the tropomyosins (TM) have not been reported. In this study, we compare the stability of raw and boiled shrimp TM of Litopenaeus vannamei and evaluate how boiling may alter the allergenicity of L. vannamei. Extracts were prepared from raw and boiled shrimp and analyzed by sodium dodecyl sulfate-polyacrylamide gel electr...
Isolation of cDNA clones coding for IgE autoantigens with serum IgE from atopic dermatitis patients.
Recently we demonstrated that a high percentage of atopic dermatitis (AD) patients displayed specific immunoglobulin E reactivity to human proteins. Here we show that IgE autoreactivity is found predominantly in AD patients with severe skin manifestations and reveal the molecular nature of four IgE autoantigens. An expression cDNA library constructed from a human epithelial cell line (A 431) wa...
The high incidence of food allergies, including oral allergy syndrome, represent major considerations when introducing new crops and foods. A new structural database of allergenic proteins, SDAP-Food, http://fermi.utmb.edu/SDAP/, has been developed to aid in predicting the IgE-binding potential of novel food proteins and cross-reactivities among known allergens. The site is designed to facilita...
BACKGROUND Acid-hydrolyzed wheat proteins (acid-HWPs) have been shown to provoke severe allergic reactions in Europe and Japan that are distinct from classical wheat allergies. Acid-HWPs were shown to contain neo-epitopes induced by the deamidation of gluten proteins. However, products with variable rates of deamidation can be found. OBJECTIVES In this work, we studied the effect of the exten...
Wheat is an important staple food and potent allergen source. Recently, we isolated a cDNA coding for wheat alpha-purothionin which is recognized by wheat food allergic patients at risk for severe wheat-induced allergy. The purpose of the present study was the biochemical, biophysical and IgE epitope characterization of recombinant alpha-purothionin. Synthetic genes coding for alpha-purothionin...
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