Important biological consequences are related to the reaction of HO• radicals with methionine (Met). Several fundamental aspects remain to be defined when Met is an amino acid residue incorporated in the interior of peptides and proteins. The present study focuses on Gly-Met-Gly, the simplest peptide where Met is not a terminal residue. The reactions of HO• with Gly-Met-Gly and its N-acetyl der...

The amidation of a synthetic peptide D-Tyr-Pro-Gly-Gly by sheep hypothalamic and pituitary preparations was measured. This substrate was designed as a glycine-extended C-terminal peptide analogue of gonadotropin-releasing hormone (GnRH) to test the ability of these tissues to convert the product produced by cleavage of the GnRH prohormone into the active amidated decapeptide. An alpha-amidating...

Objective(s): Propofol (2, 6-diisopropylphenol) is an intravenous anesthetic that is commonly used for the general anesthesia. It is well known that the spinal cord is one of the working targets of general anesthesia including propofol. However, there is a lack of investigation of the effects of propofol on spinal dorsal horn which is important for the sensory transmission of nociceptive signal...

Placenta is a special organ that contains many nutrients such as growth factors, minerals, and bioactive peptides. Dipeptides of glycine and leucine are major components of porcine placenta extracts (PPE) that has been used as an alternative of human placenta extracts. In this study, we investigated whether major peptides of PPE, Glycyl-L-Leucine (Gly-Leu), L-Leucyl-Glycine (Leu-Gly), and L-Leu...

We evaluated seizure profile, Toll-like receptor (TLR)-4 polymorphisms, and serum matrix metalloproteinases (MMPs) in patients with calcified neurocysticercosis.One-hundred nine patients with calcified neurocysticercosis with newly diagnosed seizures and 109 control subjects were enrolled. TLR-4 Asp299Gly and Thr399Ile polymorphisms and serum MMP-9 levels were evaluated. The patients were follo...

In this study, orodispersible films formed from hydroxypropyl methylcellulose (HPMC) E6 (2, 2.5, and 3%) plasticizers ((glycerin (Gly), propylene glycol (PP), or polyethylene (PEG)), containing doxazosin mesylate, were prepared by the solvent casting method characterized. Design of experiments (DoE) was used as a statistical tool to facilitate interpretation experimental data allow identificati...

In the present work, multi-walled carbon nanotubes (MWCNTs) were used as support material for impregnation of metallic nanoparticles (MNPs) produced by green synthesis. The influences plant extracts (pomegranate (Punica Granatum), Eucalyptus, and pecan (Carya illinoinensis, leaves), metal species (copper iron), concentrations, type functionalization (OH COOH) on characteristics obtained materia...

The flux of the glycine residue of glycyl-L-proline (GlyPro) from intestinal lumen into mucosal cell was investigated in rabbit ileum. Measurements of tissue W, after 60-s exposure of the mucosal surface to [Gly-W]Gly-Pro at concentrations from 0.05 to 32 m, suggest two saturable influx processes, one with a V,,, of 0.59 pmole per cm2 hr and an apparent affinity constant (K,) of 0.93 II~M, and ...

Current models of the virus-like agents of scrapie and bovine spongiform encephalopathy (BSE) have to take into account that structural changes in a host-encoded protein (PrP protein) exhibit an effect on the time course of these diseases and the survival time of any man or animal exposed to these pathogens. We report here the sequence of different forms of the bovine PrP gene which contain eit...