this study was conducted to optimize a highly efficient mrna transfection into dendritic cells (dc) derived from esophageal squamous cell carcinoma (escc) patients. applying an electroporation technique, in vitro synthesized green fluorescent protein (gfp) mrna was transfected as an indicator into the dcs derived from a healthy donor. flow cytometry revealed 84.9% transfection efficiency for dc...

Green fluorescent protein (GFP) is a reporter widely utilized to evaluate gene transfer efficiency and used as control in therapeutic overexpression strategies. Although it frequently considered be an innocuous protein, there also evidence indicating interference with contractile function electrophysiological properties, potentially leading cardiac dilation functional deterioration. Cognizant o...

Numerous genes involved in calcification, algal endosymbiosis, and the stress response have been identified corals by large-scale gene expression analysis, but functional analysis of those is lacking. There are few experimental examples manipulation corals, such as knockdown RNA interference (RNAi). The purpose this study to establish an RNAi method for coral juveniles. As a first trial, encodi...

Abstract Objective The controversial General Factor of Personality (GFP) has been proposed as an indicator social effectiveness and a slower life history strategy. An alternative hypothesis holds that only meta-trait alpha , comprising agreeableness, conscientiousness, emotional stability, is slow indicator. This study tested whether the GFP and/or emerges from both self- stranger-ratings, eith...

Objective: Myh11 encodes a myosin heavy chain protein that is specifically expressed in smooth muscle cells (SMCs) and important for maintaining vascular wall stability. The goal of this study to generate dual reporter mouse line definitive visualization MYH11 + SMCs vivo. Approach Results: We generated knock-in model by inserting LoxP-nlacZ-4XpolyA-LoxP-H2B-GFP-polyA-FRT-Neo-FRT cassette into ...