Limited comprehensive molecular typing data exist currently for Panton-Valentine leucocidin (PVL)-positive, methicillin-sensitive Staphylococcus aureus (PVL-MSSA) clinical isolates. Characterization of PVL-MSSA isolates by multilocus sequence typing (MLST) and spa typing in this study showed a genetic similarity to PVL-positive, methicillin-resistant S. aureus (PVL-MRSA) strains, although three...

Sequence-based epidemiological typing of methicillin-resistant Staphylococcus aureus (MRSA) has recently been promoted because it results in unambiguous data sets that can be organized in local and global databases. The replacement of previous typing methods, such as the highly discriminatory pulsed-field gel electrophoresis (PFGE), has been attempted with various markers and typing schemes, in...

conclusions the outcome of this study indicates a higher discriminatory power of the rflp analysis based on the spa gene compared to the coa gene. moreover, the results of our study reveal that the resistance rate of s. aureus to some antimicrobial agents including linezolid is a growing concern. background antibiotic resistant staphylococcus aureus is a serious public health problem worldwide....

Forensic genetics, leveraging molecular tools and scientific applications, has witnessed significant advancements in DNA analysis over the last three decades. These progressions have enhanced discrimination power, speed, sensitivity of profiling methods, enabling challenging samples. This article explores significance forensic genetics criminal investigations, traces historical evolution techni...

BackgroundStreptococcus pneumoniae (S. pneumoniae) of serogroup 19 are mainly represented by serotypes 19A and 19F, which associated with antimicrobial resistance disease. The wzy gene, a component the pneumococcal capsular locus, is target to differentiate 19F PCR-based typing. In last decade, allelic variants wzy19F gene have been described, leading misinterpretation typing results.MethodsA c...

Mobile genetic elements (MGEs) and genetic rearrangement are considered as major driving forces of bacterial diversification. Previous comparative genome analysis of Porphyromonas gingivalis, a pathogen related to periodontitis, implied such an important relationship. As a counterpart system to MGEs, clustered regularly interspaced short palindromic repeats (CRISPRs) in bacteria may be useful f...