The Dwarf Sunflower, Helianthus annuus, is a hyperaccumulator of the heavy metals cadmium, nickel, and chromium. The molecular mechanism of hyperaccumulation and adapting to the resulting abiotic stress is largely unknown. Metal-binding and other proteins induced in response to stress conditions may play indispensable roles in allowing the metals to accumulate and the plant to adapt itself. How...

Glucose oxidase is an active oxygen species generating enzyme produced from Aspergillus niger grown in submerged fermentation. Disintegration of the mycelium resulted in high glucose oxidase activity that was subjected to ammonium sulfate precipitation at 60-85% saturation rates that resulted to 6.14 U mg specific activity. Purification of enzyme by anion exchange column (DEAE-Cellulose) result...

A thiamine-binding protein was purified from the extract of rice bran acetone powder by conventional procedures of acid precipitation, a series of column chromatography on DEAE-Sephadex A-50 and DEAE-cellulose, and gel filtration of Sephadex G-200. The purified thiamine-binding protein was nearly homogeneous as judged by disc gel electrophoresis and the molecular weight was estimated to be 94,0...

INTRODUCTION Previous numerous papers by the senior author dealt with the human serum factor referred to as anti-antibody which is specifically directed against IgG antibodies that underwent molecular transformation in the course of the reactions with their corresponding antigens. The reactions of this serum factor could be conveniently detected by means of agglutination of Rh-positive erythroc...

I. A unique hemoprotein has been isolated from a hemolysate of normal human erythrocytes by a procedure which involves (NH4),SO 4 precipitation and chromatography on Amberlite CG-5o, DEAE-cellulose, Bio-Gel P-6o, and Bio-Gel P-3o. 2. The purified hemoprotein appeared homogeneous on ultracentrifugation although small amounts of impurities were detected by polyacrylamide gel electrophoresis. The ...

Sera from nine out of 30 (30.0%) apparently healthy unvaccinated indigenous (Tswana) chickens had precipitating antibodies to infectious bursal disease (IBD) virus using the agar gel precipitation test. Similarly, sera from 11 out of 49 (22.4%) chickens of exotic breeds with no history of vaccination against IBD were positive for antibodies against the virus.

In the present investigation, protein extraction protocol for biofilm forming strain of P. aeruginosa has been optimized. It was observed that conventional TCA/Acetone precipitation method was insufficient for 2D PAGE analysis. So, a slight modification in protein extraction protocol has been made to make it suitable for 2D PAGE analysis. This protocol appears to be suitable for 2D PAGE analysi...

Two strains of Mycoplasma were isolated from synovial fluids of arthritic feeder cattle and were identified as Mycoplasma agalactiae subsp. bovis by growth inhibition and fluorescent antibody tests. The strains (Iowa 1136 and Nebraska 2) could not be distinguished from known strains (Donetta and California 01) by immunoelectrophoresis or by agar gel precipitation.

Prolactin presents important size heterogeneity in terms of circulating molecular forms. Besides the monomer (23kDa), which predominates in healthy subjects as well as in patients with prolactinoma, high molecular forms are also present in low concentrations (1). These high molecular forms include 50 kDa dimers (big prolactin) and also forms with even larger molecular weights (MW>150 kDa), know...

Marek’s disease (MD) was observed in Japanese quails. Gross lesions were confined mostly to the proventriculus, spleen and liver. Microscopic lesions were commonly seen in the spleen and liver. Suggestive lesions of MD were observed in seven out of 20 birds grossly. Organs were collected from all the 20 necropsied birds. The birds which were not revealing gross lesions were also tested for MD v...