نتایج جستجو برای: explant technique
تعداد نتایج: 614595 فیلتر نتایج به سال:
PURPOSE To describe the phenotypic characteristics of a limbal epithelial cell sheet outgrowth from a limbal explant cultured on amniotic membrane. METHOD Immunofluorescent staining and confocal microscopy were used to examine the expressions of p63, Ki-67, keratins 3 and 14, connexin 43, and the integrin alpha6/beta4 and alpha3/beta1 subunits in corneal and limbal tissues in a limbal explant...
The medium pH level of plant tissue cultures has been shown to be essential to many aspects of explant development and growth. Sensitivity or tolerance of medium pH change varies according to specific requirements of in vitro individual species. The objectives of this study are to 1) determine medium pH change over time in storage conditions and with presence of explants, 2) evaluate the effect...
Several studies have shown that decidual tissue produces soluble factors which can mediate a variety of immunosuppressive activities. In order to assess whether prostaglandin E2 (PGE2) was present in supernatants produced by explant culture of 10 human specimens of first trimester decidua. The effect of the supernatants from the two different series (with and without indomethacin treatment) ...
Objective: To compare the characteristics of human gingival fibroblasts (hGFs) obtained by tissue-explant and enzyme-digestion methods. Methods: The hGFs were isolated using tissue explants and enzyme digestion methods and identified by immunocytochemical analysis. Cell-surface molecules were detected by flow cytometry analysis. The colony-forming activity was examined by CFU-F assay. Oil red O...
BACKGROUND Insulin-like growth factor 1 (IGF-1) activates prosurvival pathways and improves postischemic cardiac function, but this key cytokine is not robustly expressed by cultured human cardiac stem cells. We explored the influence of an enhanced IGF-1 paracrine signature on explant-derived cardiac stem cell-mediated cardiac repair. METHODS AND RESULTS Receptor profiling demonstrated that ...
Objective The purpose of this study was to investigate the effects of estradiol on the expression of hypoxia-inducible factor (HIF)-1α and the differentiation of trophoblasts in human first trimester villous explant cultures. Methods Villous explant cultures were established from first trimester human placentas (6-8 weeks of gestation, n=3). Normal villous tissues were explanted on Matrigel a...
BACKGROUND Dexamethasone (DEX) is used for the treatment of various inner ear diseases. However, the molecular mechanism of DEX on gentamicin induced hair cell damage is not known. Therefore, this study investigated the protective effect of DEX on gentamicin (GM)-induced ototoxicity and the effect of GM on the expression of apoptosis related genes. METHODS The protective effects of DEX were m...
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