نتایج جستجو برای: esat 6 protein

تعداد نتایج: 2065964  

2011
Kathryn L. Kellar Jennifer Gehrke Stephen E. Weis Aida Mahmutovic-Mayhew Blachy Davila Margan J. Zajdowicz Robin Scarborough Philip A. LoBue Alfred A. Lardizabal Charles L. Daley Randall R. Reves John Bernardo Brandon H. Campbell William C. Whitworth Gerald H. Mazurek

BACKGROUND Mycobacterium tuberculosis (Mtb) infection may cause overt disease or remain latent. Interferon gamma release assays (IGRAs) detect Mtb infection, both latent infection and infection manifesting as overt disease, by measuring whole-blood interferon gamma (IFN-γ) responses to Mtb antigens such as early secreted antigenic target-6 (ESAT-6), culture filtrate protein 10 (CFP-10), and TB7...

2014
F. Song X. Sun X. Wang Y. Nai Z. Liu

BACKGROUND Early diagnosis of tuberculous meningitis (TBM) is still a challenge; the present study aimed to establish a method of detecting the antigen early secreted antigenic target 6 (ESAT-6) in cerebrospinal fluid (CSF) by an indirect enzyme-linked immunosorbance assay (ELISA) protocol and to study the value of detecting ESAT-6 in CSF in the early diagnosis of TBM. METHODS An indirect ELI...

2018
Takahiro USHIGUSA Yoshiyuki KOYAMA Tomoko ITO Kenichi WATANABE James K. CHAMBERS Aya HASEGAWA Kazuyuki UCHIDA Ryoji KANEGI Shingo HATOYA Toshio INABA Kikuya SUGIURA

By using a complex of DNA, polyethylenimine and chondroitin sulfate, the in vivo transfection of early secretory antigenic target-6 (ESAT-6) gene into tumor cells was found to cause significant suppression of the tumor growth. In order to apply the method in clinical cancer treatment in dogs and cats, mechanisms underlying the suppressive effects were investigated in a tumor-bearing mouse model...

ژورنال: :مجله میکروب شناسی پزشکی ایران 0
مرصع فرناد morasa farnad islamic azad university, zanjan branche, zanjan, iranدانشگاه آزاد اسلامی، واحد زنجان حسین خان احمد شهررضا hossein khanahmad shahreza pasteur institute of iran, tehran, iranمجتمع تولیدی تحقیقاتی انستیتو پاستور ایران بهمن تبرائی bahman tabaraee pasteur institute of iran, tehran, iranمجتمع تولیدی تحقیقاتی انستیتو پاستور ایران بهادر یزدان پناه bahador yazdan pana pasteur institute of iran, tehran, iranمجتمع تولیدی تحقیقاتی انستیتو پاستور ایران حسام موثق hesam movassagh pasteur institute of iran, tehran, iranمجتمع تولیدی تحقیقاتی انستیتو پاستور ایران منوچهر رستمی manochehr rostami pasteur institute of iran, tehran, iranمجتمع تولیدی تحقیقاتی انستیتو پاستور ایران طیبه سهرابی

زمینه و اهداف: در قرن گذشته تنها روش تشخیص عفونت نهفته مایکوباکتریوم توبرکولوزیس آزمایش پوستی توبرکولین بود. این آزمایش در بیماران آلوده با دیگر مایکوباکتریوم ها و در افراد واکسینه با bcg نتایج مثبت کاذب دارد. لذا، لزوم روش های تشخیصی کارآمد، سریع و دقیق مطرح می شود. دو آنتی ژن cfp-10و esat-6 در تمام مایکوباکتریوم های پاتوژن وجود دارد ولی در سویه های مولد واکسن bcgو مایکوباکتریوم های فرصت طلب ا...

2017
Morten Ruhwald Lena de Thurah Davis Kuchaka Mostafa Rafaat Zaher Ahmed M. Salman Abdel-Rahman Abdel-Ghaffar Faten Aly Shoukry Sascha Wilk Michelsen Bolette Soborg Thomas Blauenfeldt Stellah Mpagama Søren T. Hoff Else Marie Agger Ida Rosenkrands Claus Aagard Gibson Kibiki Nabila El-Sheikh Peter Andersen

There is a need for an improved vaccine for tuberculosis. ESAT-6 is a cardinal vaccine antigen with unique properties and is included in several vaccine candidates in development. ESAT-6 is also the core antigen in the IFN-γ release assays (IGRA) used to diagnose latent infection, rendering IGRA tests unspecific after vaccination. This challenge has prompted the development of a companion diagn...

2016
Atefeh Seghatoleslam Mina Hemmati Saeedeh Ebadat Bahram Movahedi Zohreh Mostafavi-Pour

BACKGROUND Macrophage immune responses are affected by the secretory proteins of Mycobacterium tuberculosis (Mtb). This study aimed to examine the immune responses of macrophages to Mtb secretory antigens, namely ESAT-6, CFP-10, and ESAT-6/CFP-10. METHODS THP-1 cells (a human monocytic cell line) were cultured and differentiated to macrophages by phorbol 12-myristate 13-acetate. The cytotoxic...

Journal: :Infection and immunity 1995
A L Sørensen S Nagai G Houen P Andersen A B Andersen

A novel immunogenic antigen, the 6-kDa early secretory antigenic target (ESAT-6), from short-term culture filtrates of Mycobacterium tuberculosis was purified by hydrophobic interaction chromatography and anion-exchange chromatography by use of fast protein liquid chromatography. The antigen focused at two different pIs of 4.0 and 4.5 during isoelectric focusing, and each of these components se...

2013
Ganzhu Feng Qingtao Jiang Mei Xia Yanlai Lu Wen Qiu Dan Zhao Liwei Lu Guangyong Peng Yingwei Wang

Development of a novel and effective vaccine against Mycobacterium tuberculosis (M.tb) is a challenging for preventing TB infection. In this study, a novel nanoparticle-based recombinant DNA vaccine was developed, which contains Esat-6 three T cell epitopes (Esat-6/3e) and fms-like tyrosine kinase 3 ligand (FL) genes (termed Esat-6/3e-FL), and was enveloped with chitosan (CS) nanoparticles (nan...

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