The aim of this study was to evaluate the occurrence of peony ring spot disease in the collections of foreign and Lithuanian origin peony cultivars and hybrids and to identify a causal agent of this viral disease. During surveys of ornamental plant collections grown in Lithuania, solitary instances of affected by peony ring spot disease plants have been detected in all collections surveyed. Dis...

We compared complement fixation (CF) for the measurement of antibodies against influenza A, influenza B, respiratory syncytial virus (RSV), human adenovirus, and parainfluenza viruses 1, 2, and 3 (para-1, para-2, and para-3) with 2 enzyme-linked immunosorbent assays (ELISA kits, A and B). The IgG ELISA kits compared very well with each other except for the influenza A and B IgG ELISAs. The IgG ...

The aim of this study was to estimate the diagnostic value of different commercially available ELISA kits for the detection of bluetongue virus (BTV) antibodies in infected and vaccinated animals. The relative specificity of ELISA kits was evaluated using a panel of sera originating from healthy cattle, never vaccinated nor exposed to BTV. All ELISA kits applied had a high relative specificity ...

The virulence factor of Melon necrotic spot virus (MNSV), a virus that induces systemic necrotic spot disease on melon plants, was investigated. When the replication protein p29 was expressed in N. benthamiana using a Cucumber mosaic virus vector, necrotic spots appeared on the leaf tissue. Transmission electron microscopy revealed abnormal mitochondrial aggregation in these tissues. Fractionat...

Gentiana species generally are of little importance to horticulture. This may explain that little attention has been given to virus infections in these ornamentals, so far. However, during the last couple of years the number of samples for virus diagnosis increased. The main symptoms recorded, were growth reduction, necrotic lesions and decline. The virus isolated most frequently was broad bean...