A multiplex PCR assay was developed which allowed the simultaneous detection of five trait genes or virulence markers in enterohemorrhagic Escherichia coli (EHEC) serotypes. A primer pair, designed to detect a single base-pair mutation in the uidA gene, is specific only for the prototypic EHEC of O157:H7 serotype and its toxigenic, non-motile variants. In a similar way, primers to the eaeA gene...

A DNA piezoelectric biosensing method for real-time detection of Escherichia coli O157:H7 in a circulating-flow system was developed in this study. Specific probes [a 30-mer oligonucleotide with or without additional 12 deoxythymidine 5'-monophosphate (12-dT)] for the detection of E. coli O157:H7 gene eaeA, synthetic oligonucleotide targets (30 and 104 mer) and PCR-amplified DNA fragments from ...

The present study was conducted to detect the occurrence, serotype, genotype, phylogenetic relationship and antimicrobial resistance pattern of STEC from healthy goats of West Bengal, India. From the 125 faecal samples collected from healthy goats, 245 isolates were identified as Escherichia coli. The E. coli harbouring any gene for Shiga toxins (stx1/stx2) was detected in 36 (14.7%) of the 245...

AIM This study was planned to study the genotypes of verocytotoxigenic Escherichia coli (VTEC) in fecal samples of deer due to its public health significance. MATERIALS AND METHODS A total of 160 fecal samples of deer were taken from Mathura district and Kanpur Zoo and screened for VTEC genes by polymerase chain reaction (PCR). RESULTS All fecal samples were positive for E. coli. All the E....

The present study investigates the antioxidant potential of the ethyl acetate fraction of the aerial parts Eclipta alba L. Hassk (EAEA) which was widely used as hepatoprotective plant. EAEA was orally administered at doses of 50, 100 and 200 mg/kg for 7 days in male Charles Foster rats. The extent of hepatoprotective potential of E . alba was studied by assessing the biochemical para...

This study designed to isolation virulence E. coli, specific phages and identify in rabbits biochemically, serologically, detect genes using PCR susceptibility antimicrobial range. For propose fifty-fecal-swab samples collected from diarrheic freshly-dead-young-rabbits were presented bacteriological examination. Data showed twenty-seven (54%) gave positive results of Escherichia coli. in-vitro ...