We report a case of tectonic corneal transplantation for impending corneal perforation to preserve anatomic integrity using cryopreserved donor tissue. An 82-year-old woman exhibiting impending corneal perforation suffered from moderate ocular pain in the left eye for one week. After abnormal tissues around the impending perforation area were carefully peeled away using a Crescent blade and Van...

Six human donor corneas were studied with the scanning electron microscope to quantify the hazards to the endothelium during the excision of corneoscleral buttons. Although the number studied was small, it was found that: (1) striae were more numerous in the flaccid, very young, donor corneas (under 1 year old) than in the more rigid adult corneas: (2) iridocorneal endothelial touch can result ...

An improved method of banking the donor material at low temperature is described, entailing the immersion of the cornea with an attached rim of sclera in medium containing dimethyl sulphoxide. The storage temperature is that of liquid nitrogen (1960 C.) instead of that of solid carbon dioxide (79° C.), as formerly used. Though the results with deep-frozen donor material were superior to those w...

Corneas with severe pathologies have a high risk of rejection when conventionally grafted with human donor tissues. In this early observational study, we grafted bioengineered corneal implants made from recombinant human collagen and synthetic phosphorylcholine polymer into three patients for whom donor cornea transplantation carried a high risk of transplant failure. These patients suffered fr...

Current treatments for reversible blindness caused by corneal endothelial cell failure involve replacing the failed endothelium with donor tissue using a one donor-one recipient strategy. Due to the increasing pressure of a worldwide donor cornea shortage there has been considerable interest in developing alternative strategies to treat endothelial disorders using expanded cell replacement ther...