A new analytical approach for the detection of Ni2+ utilizing an attenuated total reflection (ATR) technique is discussed in this paper. Nickel detection was accomplished on a silicon ATR parallelogram crystal uniformly coated by a ca. 1.5-microm Nafion film embedded with dimethylglyoxime (DMG) probe molecules. The detection of Ni2+ is based on the appearance of a unique infrared absorption pea...

Most of the methods described for the determination of acetoin and biacetyl are based on the original Lemoigne-van Niel procedure (1, 2), which depends upon the formation of insoluble nickel dimethylglyoxime when biacetyl is treated with hydroxylamine in the presence of a nickel salt; acetoin is similarly determined after oxidation to biacetyl by heating with ferric chloride. In a recent modifi...

A procedure whereby reflectance spectroscopy can be employed for the analysis of substances resQlved on thinlayer plates was developed with the use of metal cation mixtures that had been separated on cellulose plates. A rapid method was devised whereby fourteen cations were separated and identified ~~ by their reflectance spectra after chromogenesis with a mixture of dithizone and 8-hydroxyquin...

The specificities of phosphate donors and the effects of metal chelating agents and divalent metal ions on NAD kinase activation by phytochrome-far red-absorbing form (Pfr) were examined. ATP was the most efficient phosphorylating agent. Uridine 5'-triphosphate, cytidine 5'-triphosphate (CTP), inosine 5'-triphosphate, and guanosine 5'-triphosphate in this order caused significant phosphorylatio...

In order to decrease the burden of contact allergy, release of nickel from products intended for direct and prolonged contact with the skin has since the year 2000 been limited to 0.5 µg Ni/cm 2 /week in the European Union. This is according to the former EU Nickel Directive, which is part of the EU chemicals regulation REACH since 2009 (1). The restriction has resulted in some reduction of the...

A commercial and disposable screen-printed carbon electrode (SPCE) has been proposed for a fast, simple low-cost determination of Ni(II) at very low concentration levels by differential pulse adsorptive stripping voltammetry (DPAdSV) in the presence dimethylglyoxime (DMG) as complexing agent. In contrast with previously methods, Ni(II)-DMG complex adsorbs directly on surface, no need mercury, b...