نتایج جستجو برای: dilution end point 10
تعداد نتایج: 1819129 فیلتر نتایج به سال:
Panoptic segmentation has recently unified semantic and instance segmentation, previously addressed separately, thus taking a step further towards creating more comprehensive efficient perception systems. In this letter, we present Panoster, novel proposal-free panoptic method for LiDAR point clouds. Unlike previous approaches relying on several steps to group pixels or points into objects, Pan...
Point of interest (POI) data serves as a valuable source semantic information for places and has many geospatial applications in real estate, transportation, urban planning. With the availability different sources, POI conflation technique enriching quality coverage by merging from multiple sources. This study proposes novel end-to-end framework consisting six steps, starting with procurement, ...
This paper reports a reconfigurable microfluidic dilution device for high-throughput quantitative assays, which can easily produce discrete logarithmic/binary concentration profiles ranging from 1 to 100-fold dilution in parallel from a fixed sample volume (e.g., 10 μL) without any assistance of continuous fluidic pump or robotic automation. The integrated dilution generation chip consists of s...
The 'Random Mutation Capture' assay allows for the sensitive quantitation of DNA mutations at extremely low mutation frequencies. This method is based on PCR detection of mutations that render the mutated target sequence resistant to restriction enzyme digestion. The original protocol prescribes an end-point dilution to about 0.1 mutant DNA molecules per PCR well, such that the mutation burden ...
The "common" 4977 bp deletion in mitochondrial DNA (Delta4977) is commonly used as an indicator of tissue deterioration in ageing and bioenergetic diseases. Deletion levels are normally measured by a serial dilution polymerase chain reaction (PCR) approach, where test reactions are compared with dilutions of control amplifications of DNA from a similar sized stable region of the mitochondrial g...
A growing number of learners join HE institutions through degree apprenticeships with a strong emphasis on both on-the-job and off-the-job training, apprentices sharing time between lecture theatres the workplace. In addition to meeting requirements, completion requires passing work-based end-point assessment (EPA). EPAs often include capstone project that is equivalent or dissertation plays cr...
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